苯那普利后处理与缺血后处理对离体心脏的保护作用  

Protective Effect of Bennazepril and Ischemic Postconditioning in Isolated Heart

作　　者：吴慧颖[1] 唐丽君 张英杰[3] 

机构地区：[1]商丘市第一人民医院心血管内科,商丘476000 [2]商丘市医学高等专科学校,商丘476000 [3]辽宁医学院附属第一医院心内科,锦州121000

出　　处：《中国医药导刊》2009年第8期1348-1349,1360,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:观察离体状态下,苯那普利后处理与缺血后处理是否均具有减轻心肌缺血再灌注损伤(IRI)的作用,并初步探讨其可能的作用机制。方法:应用Langendroff离体灌流装置,采用完全停灌-复灌的方法制作离体大鼠心肌缺血再灌注模型。将32只SD大鼠随机等分为4组,缺血再灌注组(Ⅰ),缺血预处理组(Ⅱ),缺血后处理组(Ⅲ)和苯那普利后处理组(Ⅳ)。测定各组再灌注60min时的心脏流出液中一氧化氮(NO)的含量,改良亮绿变色酸法(GCA)观察心肌损害程度,免疫组化法检测心肌组织中核因子-κB(NF-κB)和肿瘤坏死因子(TNF-α)的表达。结果:Ⅱ、Ⅲ和Ⅳ三组再灌注60min时心脏流出液中NO含量分别为(102.8±6.9)ml/min、(102.5±6.1)ml/min、(101.7±8.3)ml/min,均明显高于Ⅰ组[(27.8±5.9)ml/min,P<0.01];Ⅱ、Ⅲ和Ⅳ三组的心肌损害阳性面积分别为(13.1±7.1)%、(17.4%±9.1)%、(14.0±7.2)%,均明显低于Ⅰ组[(40.4±16.9)%,P<0.01]:Ⅱ、Ⅲ和Ⅳ三组的NF-κB阳性表达率分别为(33.53±12.19)%、(32.7±5.57)%、(34.77±4.66)%,均明显低于Ⅰ组(49.35±9.74)%,P<0.01];Ⅰ组的TNF-α表达呈强阳性。而Ⅱ、Ⅲ和Ⅳ三组的TNF-α表达均为弱阳性:Ⅱ、Ⅲ和Ⅳ三组间比较,差异无统计学意义(P>0.05)。结论:苯那普利后处理与缺血后处理均具有减轻心肌缺血再灌注损伤作用,其机制可能与升高NO含量,抑制NF-κB的表达,从而减轻炎症反应有关。Objective： To observe the protective effects of Benazepril postconditioning and ischemic postconditioning in isolated rats hearts, and investigate the possible mechanism. Methods： Thirty-two male Sprague-Dawley rats were isolated and perfused using the Langendorff mode with modified Kreb-Henseleit （KH） buffer and were randomly assigned into four groups： I/R group, preconditioning group, postconditioning group, B enazepril postconditioning group. The level of nitric oxide （NO） in the coronary effluent by spectrophotometry at the minute of reperfusion 60 minute, myocardial infarction size was examined by Gonori chromotropie acid staining （GCA） .The NF-κB and TNF-α were investigated by immunohistochemical technique. Results：The level of NO in the coronary effluent at 60 min of reperfusion was significantly increased in preconditioning group（102.8±6.9ml/min, P〈0.01） postconditioning group[ （102.5±6.1） ml/min, P〈0.01], and Benazepril postconditioning group（101.7±8.3） ml/min, P〈0.01）,as compared to I/R group （27.8±5.9） ml/min ; Myocardial infarction size was significantly reduced in preconditioning group （13.1%±7.1%, P〈0.01）, postconditioning group（17.4%±9.1%, P〈0.01） and Benazepril posteonditioning group（14.0%±7.2%, P〈0.01） as compared to I/R group （40.4%±16.9%）; The expression of NF-κB was less in preconditioning group（33.53%±12.19%, P 〈0.01）, postconditioning group （32.78%±5.57%, P〈0.01 ） and Benazepril postconditioning group（34.77%±4.66%, P〈0.01） ,than that in I/R group （49.35%±9.74%）; The expression of TNF-α was strong positive reaction in I/R group ,The expression of TNF-α was weak positive reaction in Benazepril postconditioning group, postconditioning group and preconditioning group. There was no statistically significant among Benazepril postconditioning group, preconditioning group and postconditioning group （P〉0.05）. Conclusion： Benazepril postconditinning and ischemic postconditioning can protect

关 键 词：苯那普利 后处理 缺血再灌注 心肌 

分 类 号：R96[医药卫生—药理学]