双歧三联活菌片对甲氨蝶呤所致大鼠黏膜炎的干预作用  

Effect of Bacillus Bifidus on Mucositis Induced by Methotrexate in Rats

作　　者：宋丽[1] 陈力军[1] 王红美[1] 戴云鹏[1] 

机构地区：[1]山东大学附属省立医院小儿血液科,济南250012

出　　处：《实用儿科临床杂志》2009年第15期1176-1178,共3页Journal of Applied Clinical Pediatrics

基　　金：山东省卫生厅立项课题(2007HZ073)

摘　　要：目的探讨双歧三联活菌片(金双歧)防治甲氨蝶呤(MTX)所致Wistar大鼠肠黏膜炎的机制和疗效。方法Wistar大鼠54只随机分为正常对照组(A组)、MTX损伤组(B组)及金双歧干预组(C组)3组。B、C组每天予1次MTX100mg/kg(9g/L盐水稀释成1mL)腹腔注射,C组于注射MTX前2d开始每日早晚2次各喂食金双歧1粒。于造模成功第1、3、5天各处死6只大鼠,采用免疫组织化学法检测大鼠肠黏膜组织中TNF-α表达;ELISA法检测其肠黏膜表面黏液中分泌性IgA(sIgA)表达;对其肠系膜淋巴结进行细菌培养以观察大鼠肠道细菌移位,并在HE染色下观察其小肠组织形态学变化及各组大鼠死亡情况。结果C组大鼠肠黏膜组织形态较B组明显改善,绒毛高度和隐窝深度在第1、3、5天均高于B组(Pa<0.05);上皮细胞中TNF-α表达轻于B组(P<0.05);黏膜表面sIgA水平在第1、3、5天均高于B组(Pa<0.05);肠道细菌移位在第3、5天明显少于B组(Pa<0.05),大鼠病死率明显低于B组(P<0.05)。结论MTX诱发黏膜炎是一个急性损伤过程,双歧杆菌能够显著减轻大鼠肠黏膜炎性反应,缩短病程,为应用微生态制剂治疗化疗过程中所产生的不良反应提供了理论依据。Objective To explore the mechanism and therapeutic effects of bacillus bifidus on mucositis induced by Methotrexate （MTX） in Wistar rats. Methods Fifty - four Wistar rats were randomly divided into 3 groups. Group A was normal control group . All rats in group B and C received intraperitoneal injection of MTX 1 mL（100mg/kg）. The rats in group C were fed with bacillus bifidus through mouth twice each day before the 2 days of MTX injection until executed. Rats were killed on day 1,3,5 after MTX injection. Then tumor necrosis factor-α （TNF -α） was detected on intestines by means of immunohistoehemistry. Secreted immunoglobulin（sIgA） was detected on intestinal mueosal fluid by enzyme linked immunosorbent assay. Bacterial transloeation from intestine was detected by bacterial culture of mesenterie lymph node. The change of tissue morphology and the mortality were observed. Results Compared with group B, the change of tissue morphology had improved significantly in group C, villus height and crypt depth were higher significantly on 1,3,5 day（P 〈 0.05 ） ; the expression of TNF - α in endothelial cell was decreased significantly （ P 〈 0.05 ） ; sIgA in intestinal mucus increased significantly （ P 〈 0.05 ） , and bacterial translocation decreased significantly on 3,5 day（P 〈 0.05 ）. In the end,it degraded the mortality （ P 〈 0.05 ）. Conclusions The MTX - induced mueositis is an acute impairing procedure. Bacillus bifidus can mitigate mueositis obviously, and shorten the course of disease. It provided a theoretical basis for the administration of bifidobacteria to prevent the side effects in chemotherapy.

关 键 词：双歧杆菌 黏膜炎 甲氨蝶呤 不良反应 

分 类 号：R595.3[医药卫生—内科学]