CD4^+CD_(25)^+、CD8^+CD_(28)^-调节性T细胞在哮喘患儿中的变化及其临床意义  

Changes of CD4^+CD_(25)^+ and CD8^+CD_(28)^-Regulatory T Cells Levels in Peripheral Blood of Children with Asthma and Their Clinical Significances

作　　者：江毅[1] 张蔓莉[1] 杨在东[2] 唐亮[1] 罗树宁 熊甲赛 陆美琼 

机构地区：[1]深圳市蛇口人民医院儿科,广东深圳518067 [2]佛山市妇幼保健院儿科,广东佛山528000 [3]贵港市人民医院儿科,广西贵港537100

出　　处：《实用儿科临床杂志》2009年第16期1250-1251,1266,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的通过比较哮喘患儿外周血CD4+CD25+和CD8+CD28-调节性T细胞的变化,探讨小儿哮喘的发病机制、早期防治和减少其发生的新途径。方法采用流式细胞仪测定31例哮喘患儿急性发作期和缓解期外周血中CD4+CD25+和CD8+CD28-调节性T细胞百分率;23例健康儿童作为健康对照组。采用SPSS13.0软件进行统计学分析。结果与健康对照组及缓解期比较,哮喘急性发作期患儿外周血中CD4+CD25+和CD8+CD28-调节T细胞(3.92%±1.75%;10.95%±3.46%)均明显减低(Pa<0.01);缓解期患儿CD4+CD25+与CD8+CD28-调节性T细胞水平(5.39%±2.01%;14.37%±3.92%)仍显著低于健康对照组(6.94%±2.27%;16.07%±4.28%)(Pa<0.05)。CD4+CD25+和CD8+CD28-调节性T细胞与哮喘患儿病程呈正相关(r=0.462,0.459Pa<0.001)。结论CD4+CD25+及CD8+CD28-调节性T细胞形成的免疫网络调控异常可能是导致小儿哮喘发生、发展的重要原因。CD4+CD25+和CD8+CD28-调节性T细胞水平反映呼吸道炎性反应活动情况,与病程相关。诱导调节性T细胞的表达可望成为治疗小儿哮喘的一个新途径。Objective To compare the variation of CD4^＋ CD25^＋ and CD8^＋ CD28^-- regulatory T cell in childhood asthma, further to inves- tigate the pathogenesis of childhood asthma and explore a new way for the early prevention and treatment so as to reduce its incidence. Methods The percentages of CD4^＋ CD25^＋ and CD8^＋ CD28^- regulatory T cells in peripheral blood were detected by flowcytometry in 31 cases of childhood asthma who were in acute and alleviated period ,and 23 cases of healthy children were enrolled as healthy control group. SPSS 13.0 software was used to analyze the data. Results The levels ofCD4^＋ CD25^＋ and CD8^＋ CD28^- regulatory T cells in peripheral blood of acute asthma（3.92% ±1.75% ;10.95% ± 3.46% ） were significantly lower than those of healthy children （Pn 〈 0.01 ） ;the levels of CD4^＋ CD25^＋ and CD8^＋ CD28^-regulatory T cells in peripheral blood of alleviated asthma （5.39% ±2.01% ;14.37%±3.92% ） were significantly higher than acute childhood asthma （ Pn 〈 0.05 ）, but they were lower than those of healthy children （6.94%± 2.27 % ; 16.07 % ± 4.28 % ） （ P 〈 0.05 ）. CD4^＋ CD25^＋ and CD8^＋ CD28^- regulatory T ceils were positively correlated with the course of childhood asthma （ r = 0. 462,0. 459 P. 〈 0. 001 ）. Conclusions The regulatory abnormalities of immunologic network of CD4^＋ CD25^＋ and CD8^＋ CD28^- regulatory T cells may probably lead to the onset and the development of children asthma. The levels of CD4^＋ CD25^＋ and CD8^＋ CD28^-regulatory T cells can reflect the inflammation in the air tube, and are correlated with the course of the disease. Inducing the development of regulatory T cells can act as an effective method to treat childhood asthma, and it is expected to be a new way for treatment of childhood asthma.

关 键 词：CD4^+ CD25^+、CD8^+ CD28^- 调节性T细胞 哮喘 儿童 

分 类 号：R725.6[医药卫生—儿科]