保肝复功水煎剂对急性肝损害大鼠肝组织Toll样受体2、4表达的影响  

Effect of Baogan Fugong decoction on gene expression of TLR2,TLR4 in liver tissue of rats with experimental hepatitis induced by lipopolysaccharides

作　　者：陈蔓芙[1] 吴洪斌[1] 曾浩[1] 代琳[1] 朱胜[1] 许丹[1] 喻媛媛[1] 

机构地区：[1]武汉市中医医院,湖北武汉430049

出　　处：《中西医结合肝病杂志》2009年第4期224-226,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：武汉市科技局资助项目(No.200860923432)

摘　　要：目的:观察保肝复功水煎剂对急性肝损害大鼠肝脏Toll样受体(TLR)2、4基因表达的影响。方法:采用脂多糖(LPS)腹腔注射制作大鼠急性肝损害模型,将60只大鼠分为正常组、模型组及保肝复功水煎剂低、中、高剂量组。RT-PCR检测肝组织TLR2、TLR4基因表达水平。结果:模型组TLR2、TLR4基因相对表达量均明显高于正常组(P<0.05);保肝复功水煎剂中、高剂量组TLR2相对表达量明显低于模型(P<0.05);保肝复功水煎剂高剂量组TLR4相对表达量明显低于模型组(P<0.05)。结论:保肝复功水煎剂对急性肝损害具有防护作用;对TLR2、TLR4基因表达有抑制作用,这可能是其肝脏保护作用的机制之一。Objective： To investigate the effect of Baogan fugong decoction on gene expression of Toll-like receptor （TLR） 2, 4 in liver tissue of rats with experimental hepatitis induced by lipopolysaccharides （LPS） . Methods： Sixty rats were divided into five groups as follows： normal control （NC） group, model control （MC） group, Baogan Fugong low dose （BLD） group, Baogan Fugong medium dose （BMD） group, Baogan Fugong high dose （BHD） group. TLR2/4 gene expression in liver tissue of rats with experimental hepatitis induced by LPS was measured by realtime-PCR. Results： Relative gene expression of TLR2/4 in MC group were significantly higher than that in NC group （P 〈0. 05）, Relative gene expression of TLR2 in BMD and BHD group was significantly lower than that in MC group （P 〈 0. 05 ） , Relative gene expression of TLR4 in BHD group was siguificandy lower than that in MC group （P 〈 0. 05 ） . Conclusion： Up-regulation of TLR2 and TLR4 gene expression can be seen in liver tissue of rats with experimental hepatitis. Baogan Fugong decoction can inhibit the gene expression of TLR2 and TLR4. The inhibitory effects of Baogan Fugong decoction on liver damage in rats may be associated with the inhibition of TLR2 and TLR4 gene expression.

关 键 词：保肝复功水煎剂 大鼠 肝损害 TOLL样受体 

分 类 号：R285.5[医药卫生—中药学]