实时荧光定量PCR法检测乙型肝炎患者血清HBVDNA含量512例分析  

Use the methods of Real-time fluorescence quantitative PCR to detect the HBV DNA content in the patients from 312 cases of the hepatitis B patients

作　　者：黄志伟[1] 沓世念[1] 常剑锋[1] 黄德秋[1] 黄伟建[1] 沈均南[1] 陈文[1] 

机构地区：[1]肇庆市第二人民医院,526000

出　　处：《国际医药卫生导报》2009年第15期86-87,95,共3页International Medicine and Health Guidance News

摘　　要：目的探讨实时荧光定量PCR检测不同血清标志物模式的乙型肝炎患者的血清HBVDNA含量的特点。方法应用ELISA对512例病人血清中乙型肝炎血清标志物进行检测，同时用实时荧光定量PCR检测标本中HBVDNA的含量，分别统计每组的HBVDNA阳性例数及其阳性率，用u检验进行统计学处理。结果HBsAg（＋）／HBeAg（＋）／抗HBc㈩组168例，HBVDNA阳性161例，占95．8％；HBsAg（＋）／抗HBe（＋）／抗HBc（＋）组163例，HBVDNA阳性51例，占31．3％；HBsAg（＋）／抗HBc（＋）组181例，HBVDNA阳性94例占51．9％；三组之间互相比较差异均有显著性（P〈0．001）。结论HBsAg（＋）／HBeAg（＋）／抗HBc（＋）组中HBV复制最活跃，实时荧光定量PCR检测HBVDNA比血清标志更能反映乙型肝炎病毒存在与复制情况。Objective To investigate the characteristics of sertma HBV DNA content through detecting the diffent serum markers of hepatitis B using the method of the real-time fluorescence quantitative PCR.Methods 512 cases of hepatitis B serum markers were detected with ELISA, and simtdtaneously use the real-time fluorescence quantitative PCR to detect HBV DNA content in the serum. Statistics for each group of HBV DNA-positive cases and its positive rate （%） were separately done.u test were used for the statistical treatment.Results HBsAg （＋） / HBeAg （＋） / anti-HBc （＋） group has 168 cases, HBV DNA positive has 61 cases （95.8%）; HBsAg （＋） / anti-HBe （＋） / anti-HBc （＋） group has 163 cases,FIBV DNA positive has 51 cases （31.3%）; HBsAg （＋） / anti-HBc （＋） group has 181 cases,HBV DNA positive has 94 cases （51.9%）. Comparisons among three groups were significantly different （P 〈 0.001）.Conclusions The reproduction of HBV in the HBsAg （＋） / HBeAg （＋） / anti-HBc （＋） group were the most active.Real-time fluorescence quantitative PCR detection ofHBV DNA is the better method to reflect the existence and the reproduction of hepatitis B virus than Serological markers.

关 键 词：实时荧光定量PCR HBV DNA 乙型肝炎病毒 血清学标志 

分 类 号：R4[医药卫生—临床医学] R51