小麦中源于中间偃麦草抗白粉病基因PmCH5026的SSR定位  

Mapping of a Thinopyrum intermedium-derived Powdery Mildew Resistance Gene PmCH5026 in Wheat with SSR Markers

作　　者：贺润丽[1] 畅志坚[2] 刘建霞[1] 詹海仙[2] 张晓军[2] 董春林[2] 

机构地区：[1]山西大学生命科学与技术学院,山西太原030006 [2]山西省农业科学院作物遗传研究所,山西太原030031

出　　处：《山西大学学报（自然科学版）》2009年第3期455-462,共8页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(30671299;39870398);山西省科技攻关计划项目

摘　　要：CH5026是衍生于八倍体小偃麦TAI7045的新品系,用高感品种(系)CH5065、晋太170分别与CH5026配置组合,于温室接种并调查F2、F3、BC1F2群体的抗感分离之比进行遗传分析,结果表明CH5026成株期对白粉菌E09小种的抗性受1对显性基因控制,暂命名为PmCH5026.使用集群分离分析法(BSA),用378对SSR引物对CH5026×CH5065 F2代群体进行分析,筛选到标记Xcfd233、Xbarc11和Xgwm539与抗性基因连锁,位置顺序为:Xcfd233-7.2cM-PmCH5026-4.9cM-Xbarc11-5.5cM-Xgwm539.根据小麦微卫星遗传连锁图及利用中国春第2同源群缺四体、双端体对SSR标记的定位结果,将PmCH5026定位在染色体2DL上.Powdery mildew,caused by Blumeria graminis f. sp. tritici,is one of the most important diseases of wheat （Triticum aestivum L. ） worldwide and causes severe yield losses. Breeding for resistance is the most economical and effective method for controlling the disease. The wheat breeding line CH5026 derived from the partial wheat-Thinopyrum intermedium amphiploid TAI7045 is resistant to powdery mildew. To determine inheritance of powdery mildew resistance and map the resistance gene（s） in CH5026, Fz, F3, BC1F2 progenies derived from the crosses of CH5026 with line CH5065 and susceptible cv. Jintai 170 were inoculated with Bgt isolate E09 in the greenhouse. Genetic analysis indicated that a single dominant gene, temporarily designated PINCH5026, was responsible for the resistance to Bgt in line CH5026. A total of 378 SSR markers were used to test the parents and resistant and susceptible bulks from the Fz segregating population of CH5026 × CH5065. From the hulked segregant analysis,the polymorphie SSR markers were selected for genotyping the Fz population. Three SSR markers Xcfd233 ,Xbarc11 and Xgwm539 were identified to be linked to the resistance gene and their most likely order was Xcfd233,7.2 cM, PINCH5026, 4.9 eM, Xbarc11 , 5. 5 cM, Xgwm539. Using the Chinese Spring nullisomic-tetrasomic and ditelosomie lines,the SSR markers mapped the resistance gene on chromosome arm 2DL.

关 键 词：小麦 中间偃麦草 白粉病 基因定位 SSR 

分 类 号：S565.1[农业科学—作物学] S512.1