几种草坪草组织培养和植株再生的研究  

Study on the Tissue Culture and Plant Regeneration of Turfgrasses

作　　者：王慧[1] 许瑾[1] 周小梅[1] 韩玉杰[1] 张彦芹[2] 

机构地区：[1]山西大学生命科学与技术学院,山西太原030006 [2]山西省农业科学院旱地农业中心,山西太原030031

出　　处：《山西大学学报（自然科学版）》2009年第3期463-467,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省自然科学基金(2007011080)

摘　　要：以6种草坪草种子和2种草坪草愈伤组织为材料,研究了基本培养基种类和2,4-D浓度对愈伤组织诱导以及不同的细胞分裂素对分化的影响.试验结果表明,N6培养基较MS、NB培养基适合草坪草组织培养,诱导愈伤的2,4-D浓度宜采用6 mg/L,有机添加物对愈伤组织的生长有利,特别是肌醇对草坪草愈伤组织的生长有明显的促进作用.在分化培养中,不同材料所需的细胞分裂素适宜种类需要通过试验来确定.The effects of some media and concentration of 2,4-D on callus-induction with 6 kinds of Turfgrass seeds and 2 kinds of Turfgrass calli,and 3 sorts of cytokinins on callus differentiation were studied. The results showed that N6 is the optimal medium in all of the three media tested and the proper concentration of 2,4-D is 6 mg/L. The organic supplements used in subculture media, especially inositol, benefited callus growth. In differentiation culture, the suitable kinds of cytokinins needed for different materials must be decided by trial.

关 键 词：草坪草 组织培养 愈伤组织 2 4-D 有机添加物 

分 类 号：Q943.1[生物学—植物学] Q946.885