偏食高碘大鼠动物模型的建立  

Rat Model for Excessive Iodine Intake and Monophagia

作　　者：杨群[1] 郭雪芹[1] 边建朝[1] 蔺新英[1] 郭成浩[1] 

机构地区：[1]山东大学营养与食品卫生研究所,山东济南250012

出　　处：《环境与健康杂志》2009年第8期669-671,共3页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(30772134);山东省科技厅项目(2006BS03029)

摘　　要：目的建立偏食及高碘喂养的大鼠动物模型,观察其尿碘的变化规律。方法将SPF/VAF级动物Wistar大鼠随机分8组(每组8只):常规饲料组分为对照组(NC)、10倍碘组(10HI-N)、50倍碘组(50HI-N)、100倍碘组(100HI-N);偏食饲料组也分为对照组(MC)、10倍碘组(10HI-M)、50倍碘组(50HI-M)、100倍碘组(100HI-M),高碘组饮水碘含量(以I-计)分别为1400、7600、15350μg/L,每日总碘摄入量分别为对照组的10、50、100倍。每周称体重,并分别于暴露2、4和6个月测定尿碘。结果与NC组比较,各偏食高碘组体格发育迟滞。常规饲料组和偏食饲料组尿碘均随碘摄入量的增加而明显增加(P<0.01),50HI-M、100HI-M组尿碘分别高于相应常规饲料组(均为P<0.01)。结论偏食高碘大鼠生长和尿碘有明显异常,可作为有效的研究模型。Objective To observe urinary iodine levels in the rat model for excessive iodine intake and monophagia. Methods Wistar rats were randomly divided into 8 groups： normal diet control group （NC）,10-fold iodine ＋ normal diet group （10 HI-N）, 50- fold iodine ＋ normal diet group （50 HI-N）, 100-fold iodine ＋ normal diet group （100 HI-N）, monophagia control group（MC）, 10-fold iodine ＋ monophagia group （10 HI-M）, 50-fold iodine ＋ monophagia group （50 HI-M） ,100-fold iodine ＋ monophagia group （100 HI-M）. The excessive iodine intake groups exposed to 1 400, 7 600, 15 350 p^g/L I- through drinking water presented higher levels of daily iodine intake ,which were 10,50,100 times of those in control groups. During the exposure period, the body weights of the rats were measured weekly, the levels of urinary iodine were determined at the second, the fourth and the sixth month. Results The body weights of the excessive iodine intake groups with monophagia were lower than that of the NC group. In normal diet groups and monophagia diet groups ,the levels of urinary iodine increased with the increase of iodine intake. The levels of urine iodine in 50 HI-M group and 100 HI-M group were significantly higher than those of the related groups with higher iodine intake. Conclusion The experimental animal model of excessive iodine intake and monophagia are successfully established in the present research, with physical retardation and higher urinary iodine.

关 键 词：碘 偏食 尿碘 动物模型 

分 类 号：R994.6[医药卫生—毒理学]