大豆11S球蛋白基因Gy1启动子克隆及序列分析  

Cloning and nucleotide sequence analyzing of glycinin gene Gy1 promoter

作　　者：米东[1] 逯慧[1] 严琛[1] 李建粤[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2009年第4期414-417,共4页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市科学技术委员会项目(063919141)

摘　　要：以高蛋白大豆品种南农87C-38总DNA为模板,采用PCR法扩增获得约1100bp大小的DNA片段,回收该片段并克隆到pUCm-T载体上,选取阳性克隆进行PCR和酶切检测,再进行测序分析.序列分析显示该片段含1174个核苷酸,采用Vector NTI软件将试验中克隆的序列与GenBank E07850报道的Gy1启动子和GenBank X15121报道的Gy1启动子及信号肽对应序列分别进行比对,同源性分别为99.6%和99.3%,确定该片断为南农87C-38大豆11S球蛋白基因Gy1启动子及信号肽.该启动子的成功克隆,可为今后利用基因工程技术改良大豆种子营养品质研究奠定基础.About 1100bp DNA fragment of the Gyl gene promoter was amplified by PCR from genomic DNA of a Chinese high - quality soybean cuhivar Nannong 87C -38. Recovered and linked with pUCm -T vector, after using PCR and double enzymes digestion analysis, the recombinant was choosen and the sequence was identified. Comparing the cloned fragment with that of reported in GenBank EO7850 and GenBank X15121 by using vector NTI software, we found the DNA fragment composed of 1174bp nucleotide with homology 99.6% and 99.3% ,respectively. Which confirmed that the cloned fragment is the glycinin gene Gyl promoter and signal peptide. The successfully cloning glyeinin gene Gyl promoter sets up bases for improveing nutritional quality on soybean seed by gene engineering technology.

关 键 词：大豆 11S球蛋白基因 Gy1 启动子 克隆 

分 类 号：Q943.1[生物学—植物学]