HIBD新生大鼠微管相关蛋白Tau表达变化的研究  

Studies on the Level of Microtubule-associated Protein Tua in Neonatal Rats with Hypoxic-ischemic Brain Damage

作　　者：刘青[1] 张丽华[1] 杨本利[1] 王丽苹[1] 

机构地区：[1]黑龙江省小儿脑性瘫痪防治疗育中心脑瘫科,黑龙江佳木斯154003

出　　处：《中国伤残医学》2009年第4期7-9,共3页Chinese Journal of Trauma and Disability Medicine

摘　　要：目的:本研究采用经典的新生大鼠缺氧缺血性脑损伤(hypoxic-ischemicbraindamage,HIBD)动物模型,观察HIBD前后脑室周围白质Tau蛋白水平的改变,探讨新生大鼠HIBD早期Tau蛋白表达变化与脑损伤的关系。方法:清洁级7日龄Wistar大鼠82只,随机分成假手术组(对照组,n=40)和缺氧缺血性脑损伤模型组(HIBD组,n=42)。参照Rice法单侧结扎新生大鼠左颈总动脉复制HIBD动物模型;假手术组(对照组)只切开颈部正中皮肤,分离但不结扎左侧颈总动脉;HIBD～大鼠置入低氧环境缺氧2小时,而后按时间随机分为3小时、6小时、12小时、24小时、48小时组(n=8)。HE及嗜银染色后,光镜下观察HIBD前后及不同时间组新生大鼠脑神经细胞的病理变化;免疫组织化学法测各组大鼠脑室周围白质与皮层微管相关蛋白Tau水平的动态变化情况,400倍视野下利用计算机图象分析系统进行Tau蛋白平均光密度值(aver-age opticaldens卸,OD)测定,并统计学分析。结果:HIBD新生大鼠嗜银染色示脑室周围白质神经细胞中存在神经原纤维的增粗、缠绕。各实验组Tau蛋白随着缺氧缺血时间的延长参疫反应牲增强,其中H工后3小时与对照组比较差异无显著性,HIBD后6小时组、12小时组、24小时组以及48小时组的0D值较对照组明显增加(p<0.05),差异有显著性。结论:HIBD可造成新生鼠脑脑室周围白质中神经纤维变性,总Tau蛋白表达增加。Objective： This study used the classic hypoxie--ischemie brain damage（HIBD） amlimal models to observe the changes of tau protein levels in periventricular white matter before and after hypoxic--ischemic brain damage（HIBD）, and to explore the relationship between tau protein expression changes and brain damage in early neonatal rats with hypoxic- ischemic brain damage（HIBD）. Methods： Cleanly 7-- day-- old Wistar rats were randomly divided into sham operation grup （the control group, n =40） and hypoxie--ischemie brain damage model group（HIBDgroup n=42）. In the light of Rice, unilateral ligated left common carotid artery of neonatal rats to make HIBD animal model; sham operation group （control group） just cut the middle of the neck skin, isolated but not ligated the left common carotid artery; HIBD group and control group of rats were put into Hypoxie hypoxia environment 2 hours and then were randomly divided into 3h, 6h, 12h, 24h,48hgroup（n=8）. After hematoxylin and eosin staining and silver staining, using microscope observing the pathological change of neuron in newborn rats before and after HIBD at differen times;immunohistochemical observing dynamic chanaes of mierotubule--associated protein Tau level at periventricular white matter and cortical of rats, 400 times in the view, using of computer image analysis system measured the average of Tau protein optical denslty and statistcally analyzed. Results：The silver staining showed cerebral tissue with hypoxic--ischemie brain damage neonatal rats neurohbhllary tangles and aopotostie cells can be seen in periventricular alba and cerebral cortex. Comparing with the sham group, immunoreactivity of Tau in different HIBD groups was increased with prolongation of isehemia. 3 hours after HI were not signillcantly different compared with the control group,OD value of 6h group, 12h group,24h and 48h group after HIBD increased signincantly compared with the control group（p〈0. 05）, there was a signincant difference Conclution： HIBD ca

关 键 词：脑损伤 缺氧缺血性脑损伤 脑室周围白质 微管相关蛋白Tau 

分 类 号：R651[医药卫生—外科学]