壳寡糖对雌二醇诱导的乳腺癌MCF-7细胞分泌产生促肿瘤血管生成因子的抑制作用  

Inhibition effects of chitooligosaccharides on the production of pro-angiogenesis factors of E_2-induced human MCF-7 cells

作　　者：熊川男[1,2] 麻攀[1,2] 魏鹏[1,2] 徐青松[1,2] 吴海歌[1,3] 杜昱光[1] 

机构地区：[1]中国科学院大连化物所天然产物与糖工程组,辽宁大连116023 [2]中国科学院研究生院,北京100049 [3]大连大学,辽宁大连116622

出　　处：《中国海洋药物》2009年第4期1-4,共4页Chinese Journal of Marine Drugs

基　　金：国家863项目(No.2006AA100313;No.2007AA10Z343)

摘　　要：目的研究壳寡糖对雌二醇诱导的人乳腺癌MCF-7细胞分泌促肿瘤血管生成因子的抑制作用。方法采用MTT法检测处理24和48 h后壳寡糖对正常乳腺癌MCF-7细胞生长的影响;采用RT-PCR方法对MCF-7分泌促肿瘤血管生成因子MMP-9,TIMP-1,2和VEGF在mRNA水平上的变化进行考察。结果壳寡糖对没有经过雌二醇诱导的人乳腺癌MCF-7细胞的生长没有明显作用;雌二醇能够显著上调MCF-7细胞MMP-9和VEGF的mRNA水平;50μg·mL^1的壳寡糖能够显著下调由雌二醇诱导的MMP-9的mRNA水平,但是对TIMP-1,2和VEGF则没有明显作用。结论壳寡糖对雌二醇诱导的乳腺癌MCF-7细胞产生促肿瘤血管生成因子MMP-9在mRNA水平上具有显著抑制作用。Objective To investigate the inhibition effects of chitooligosaccharides on the production of pro-angiogenesis factors of estradiol （E2） induced human MCF-7 cells. Methods The cell survival rate was demonstrated by MTT assay at 24 and 48 hours; and the mRNA level of some pro-angiogenesis factors such as MMP-9, TIMP-1,2 and VEGF were investigated by RT-PCR. Results There was no significant effect of chitooligosaccharides on proliferation of human MCF-7 cells; hut when induced by E2, MMP-9 was significantly down-regulated by chitooligosaccharides. Conclusion Chitooligosaccharides had potent inhibition effects on the mRNA production of MMP-9 of human MCF-7 cells.

关 键 词：壳寡糖 雌二醇 血管生成 MMP-9 

分 类 号：R931.77[医药卫生—生药学] R965[医药卫生—药学]