可注射氯己定温度敏感水凝胶的体外释放及对牙周常见致病菌的抑制作用  

A study of injectable chlorhexidine thermosensitive hydrogel for periodontal application

作　　者：吉秋霞[1,2] 陈西广[1] 赵庆生[1] 范春[2] 

机构地区：[1]中国海洋大学,山东青岛266003 [2]青岛大学医学院附属医院,山东青岛266003

出　　处：《中国海洋药物》2009年第4期26-30,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金(30770582);国际合作重点项目(2008DFA31040);山东省卫生厅青年基金资助(2007QZ021)

摘　　要：目的研究氯己定温度敏感水凝胶的体外释放及其对牙周致病菌的抑制作用,以评价其用于牙周病治疗的可能性。方法以人工唾液为释放介质,观察氯已定在壳聚糖水凝胶中的释放度;以牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌3种牙周常见致病菌为实验菌株,测定氯己定温度敏感水凝胶对不同菌株的抑菌环直径。结果体外释放表明氯己定在人工唾液(pH6.8)中的持续释放达18h,壳聚糖温敏水凝胶有效地延缓了氯己定的释放;氯己定温度敏感水凝胶对抑菌细菌的抑菌直径范围为12～14mm,具有较强的抑制牙周常见致病菌的作用。结论氯己定壳聚糖温敏水凝胶可作为牙周袋内缓释制剂用于牙周病的治疗。Objective To evaluate the potential of chlorhexidine（Chx）-thermosensitive hydrogel as a local drug delivery system for periodontal treatment. Methods The release profiles of Chx from CS-HTCC/GP thermosensitive hydrogel in vitro were investigated. Moreover, the antibacterial activity of S-HTCC/GP-0.1 %Chx thermosensitive hydrogel to oral representative pathogens - Porphyromonas gingivalis （P. gingivalis）, Prevotella interznedia （P. intermedia ） and Actinobacillus actinomycetemcornitans （ A. actinorn yceterncomitans） was determined by inhibitory zone measurement. Results Chlorhexidine （Chx） released in vitro over 18 hours from the CS-HTCC/GP thermosensitive hydrogel in artificial saliva pH 6. 8 which more effectively retarded the release of Chx. Three periodontal pathogens were susceptible to Chx thermosensitive hydrogel with the inhibitory zone diameters over the range of 12 14mm. Conclusion All results indicated that Chx thermosensitive hydrogel can be used as a local drug delivery system for periodontal treatment.

关 键 词：温度敏感水凝胶 氯己定 体外释放 抑菌 牙周致病菌 

分 类 号：R944[医药卫生—药剂学] R965[医药卫生—药学]