甘肃丹参脂溶性成分的提取与纯化工艺研究  

Study on Technique of Extraction and Purification of Alcohol-soluble Components from Salvia Przewalskii Maxim

作　　者：杨玉琴[1] 宋平顺[2] 丁永辉 

机构地区：[1]兰州大学药学院,兰州730000 [2]甘肃省药品检验所 [3]甘肃省食品药品监督管理局

出　　处：《中国药师》2009年第9期1198-1201,共4页China Pharmacist

摘　　要：目的：研究从甘肃丹参中提取和纯化脂溶性成分的最佳工艺条件。方法：采用L9（3^4）正交设计，以总丹参酮、丹参酮ⅡA、隐丹参酮、二氢丹参酮和丹参酮Ⅰ的提取率为考察指标，优选最佳提取工艺；以总丹参酮的含量为考察指标，优选纯化总丹参酮粗提物的工艺条件。结果：总丹参酮的最佳提取条件为加8倍量的90％乙醇，回流提取3次，每次提取1h；最佳纯化工艺条件为加15倍量的5％碳酸钠溶液，洗涤5次。结论：纯化工艺条件能使总丹参酮粗提物的含量由20．31％上升至59．87％，达到了新药申报规定的要求。该提取和纯化总丹参酮的最佳工艺条件简便易行，适用于工业化生产。Objective： To optimize technical processes for extraction and purification of alcohol-soluble components from Salvia przewalskii Maxim. Method： The orthogonal design was used, the extraction percentage of total tanshinones,tanshinone Ⅱ A,cryptotanshinone.diilydrotanshinone,tanshinone Ⅰ were used as indexes, the extraction conditions of total tanshinones from Salvia przewalskii Maxim were optimized ; the extraction percentage of total tanshinones was used as indexes, the purification conditions of total tanshinones from crude extract were optimized. Result： The optimal technical process for extraction of total tanshinones was as follows ： added 8 volumes of 90% aqueous ethanol, extracted 3 times and each time for 1 hour. The optimal technical process for purification of total tanshinones was as follows： 15 volumes of 5% sodium carbonate solution, washed 5 times. Conclusion： The content of total tanshinones incrude extract increass from 20.31% to 59.87% by purification. The optimal technical processes for extraction and purification of total tanshinones are practical in industrial production.

关 键 词：甘肃丹参 总丹参酮 正交设计 提取 纯化 

分 类 号：R284.2[医药卫生—中药学]