HPLC法测定清胆胶囊中黄芩苷的含量  

Determination the Content of Baicalin in Qingdan Capsules by HPLC

作　　者：陈树和[1] 尹玲[1] 黄传俊[1] 

机构地区：[1]湖北省中医院药事部,武汉430061

出　　处：《中国药师》2009年第9期1201-1202,共2页China Pharmacist

基　　金：湖北省科技厅课题[编号:2003AA302B02]

摘　　要：目的:建立以高效液相色谱法测定清胆胶囊中黄芩苷含量的方法。方法:色谱柱Agilent HC-C_(18)(250mm×4.6mm,5μm),流动相为甲醇-0.2%磷酸(50:50),检测波长为278nm。结果:黄芩苷进样量在0.50～2.50μg范围内与峰面积积分值呈良好线性关系(r=0.999 5);平均回收率为96.13%,RSD=1.20%(n=6)。结论:本方法简便,准确可靠,可用于清胆胶囊的质量控制。Objective： To establish an HPLC method to determine the content of baicalin in qingdan capsules. Method： Agilent HC-C18 （250mm ×4.6mm,5μm） was used as chromatographic column, and the mobile phrase was consisted of methanol -0.2% phosphoric acid （50： 50） with a detection wavelength of 278 nm. Result： The linear range of Baicalin was 0.50 -2. 50μg（r = 0.999 5 ）. The average recovery of baicalin was 96.13% and RSD was 1.20% （ n = 6）. Conclusion： The method is sampe, accurate and reliable, and can be used for the quality control of qingdan capsules.

关 键 词：清胆胶囊 黄芩苷 HPLC法 含量测定 

分 类 号：R927.2[医药卫生—药学]