RP-HPLC同时测定神农香菊中绿原酸和木犀草素的含量  

Simultaneous Determination of Chlorogenic Acid and Luteolin in the Flowers of Dendranthema indicum var.aromaticum by RP-HPLC

作　　者：李志浩[1] 李鹏[1] 朱雪松[1] 郑芳[1] 朱军[1] 

机构地区：[1]郧阳医学院附属东风汽车公司总医院,湖北十堰442008

出　　处：《中国药师》2009年第9期1235-1237,共3页China Pharmacist

摘　　要：目的:建立同时测定神农香菊中绿原酸和木犀草素含量的方法。方法:采用RP-HPLC法,色谱柱为Kromasil-C_(18)(250mm×4.6mm,5μm),流动相为甲醇(A)-乙腈(B)-0.8%磷酸溶液(C),进行梯度洗脱,流速为0.5ml·min^(-1),柱温30℃,检测波长为328nm。结果:绿原酸和木犀草素分别在4.5～90.0μg·ml^(-1)(r=0.999 3)和10.5～210.0μg·ml^(-1)(r=0.999 6)浓度范围内均有良好的线性关系,平均加样回收率分别为98.72%,97.77%。结论:本方法简便,快速,准确,重复性好,可用于神农香菊药材的质量控制。Objective： To establish a method for the determination of chlorogenic acid and luteolin in the Flowers of Dendranthema indicum var. aromaticum. Method： RP-HPLC method was used with Kromasil-C18 （250mm × 4.6mm, 5μm）column and the mobile phase consisted of methanol （A）- acetonitrile （B） -0.8% natrium dihydroger phosphorate （C） with gradient elution. The flow rate was 0.5ml·min^-1, the column temperature was 30℃, and the UV detector was set at 328nm. Result： The linear ranges of chlorogenic acid and luteolin were 4.5 - 90.0μg·ml^-1 （ r = 0.999 3 ） and 10.5 - 210.0μg·ml^-1（ r = 0. 999 6）, respectively. The average recovery of chlorogenic acid and luteolin were 98.72% and 97.77%, respectively. Conclusion： The method is simple , accurate and repeatable, it can be applied in quantitative determination of chlorogenic acid and luteolin in Flowers of Dendranthema indicum var. aromaticum.

关 键 词：绿原酸 木犀草素 神农香菊 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]