BSA-PLGA缓释微球制备工艺的优化  

Optimization of preparation of BSA-PLGA controlled-release microspheres

作　　者：曾晗冰[1] 李万里[2] 徐华梓[1] 李士[1] 

机构地区：[1]温州医学院附属第二医院脊柱外科,325027 [2]浙江大学医学院附属第二医院骨科,浙江省杭州市310009

出　　处：《实用医学杂志》2009年第17期2935-2938,共4页The Journal of Practical Medicine

基　　金：浙江省自然科学基金项目(编号:Y2080507);温州市科技局项目(编号:H20080031)

摘　　要：目的:以牛血清白蛋白(BSA)为模型蛋白,乳酸/羟乙酸共聚物(PLGA)为载体,优化微球的制备工艺。方法:采用复乳溶剂挥发法制备BSA-PLGA微球,以包封率、载药量、粒径及24h释放率等为观察指标,通过正交设计法优化显著影响微球制备工艺条件的7种因素。结果:优化后的工艺条件是BSA用量为5mg、PEG用量为0.2mL、PLGA用量为200mg、PVA浓度为1%、NaCl浓度为10%、超声功率为40W、复乳搅拌速度为1000r/min。结论:该制备工艺简单、稳定,可以得到包封率高、粒径适宜、突释较少的BSA-PLGA微球。Objective To optimize the preparation technique for microspheres using BSA as model protein and PLGA as controlled-release carrier. Methods BSA-PLGA microspheres were prepared using （water-in-oil） in water emulsion solvent evaporation technique. Using entrapment efficiency, loading efficiency, particle size, and 24h-release amount as the evaluating indicators, we optimized the 7 factors that markedly influenced the preparation technique for microspheres by orthogonal factorization method. Results The optimized parameters were BSA of 5 mg, PEG of 0.2 mL, PLGA of 200 mg, 1%PVA, 10%NaCl, ultrasonic power of 40 W, and stirring rate of secondary emulsion of 1 000 r/min. Conclusions The preparation technique for BSA-PLGA microspheres is simple and stable, by which we can acquire the microspheres with high entrapment efficiency, low burst release amount, and proper particle size.

关 键 词：微球体 血清白蛋白 牛 乳酸盐类 醋酸类 聚合物 药物载体 

分 类 号：R944[医药卫生—药剂学]