水分胁迫对抗旱性不同的荔枝实生幼苗叶片质膜透性和各细胞器中Na^+-K^+ATPase活性的影响  

Effect of Water Stress on Relative Permeability of Plasma Membrane and Na^+-K^+ATPase Activities in Various Organelles of Litchi Young Seedling Leaves

作　　者：陈立松[1] 刘星辉[1] 胡又厘[1] 佘文琴[1] 

机构地区：[1]福建农业大学园艺系,福州350002

出　　处：《热带作物学报》1998年第1期58-63,共6页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金

摘　　要：以抗旱性不同的荔枝实生幼苗为试验材料，研究了水分胁迫对荔枝实生幼苗叶片质膜透性和各细胞器中Na＋－K＋ATPase活性的影响及其与抗旱性的关系，结果表明：（1）Na＋—K＋ATPase在荔枝实生幼苗叶片中的定位是细胞溶质占总活性的80．1％～82．32％，线粒体占13．34％～15．25％，叶绿体占4．34％～4．63％，品种间差异不明显。（2）叶片相对含水量随胁迫程度的增加而减少，抗旱性强的东刘1号下降的幅度小于抗旱性弱的陈紫；质膜相对透性随胁迫程度的增加而增加，抗旱性强的品种增加的幅度小于抗旱性弱的品种。（3）水分胁迫后第4天，各细胞器中Na＋－K＋ATPase活性均显著增加，抗旱性强的品种增加的幅度大于抗旱性弱的品种；8d后，抗旱性强的品种线粒体和叶绿体中Na＋－K＋ATPase继续升高，细胞溶质中Na＋－K＋ATPase呈下降趋势，但仍高于对照，而抗旱性弱的品种所有细胞器中Na＋－K＋ATPase的活性均低于对照。A Study was made to find the effect of water stress on relative permeabilityof plasma membrane and Na+-K+ATPase in various organelles of litchi(Litchi chinensisSonn. )young seedling leaves,The intracellular locations of Na+ - K+ATPase activites incytosol,mitochondria and chloroplast were measured separately and the activities werefound to be 80. 11% -82. 32%, 13. 34% - 15. 25%and 4. 34%-4. 63% respectively of thetotal activity. No significant difference was observed between varieties.The relative water content of litchi leaves declined under water stress, but less indrought resistant variety(Dongliu No-1)than in drought sensitive variety(Chenzi). The relative permeabi1ity of plasma membrane of litchi leaves increased less in drought resistantvariety than in drought sensitive variety.Four days after water stress,the Na+ -K+ATPase activities in various organelles oflitchi leaves enhanced significantly,which was higher in drought resistant variety than indrought sensitive variety;another four days of water stress showed that the Na＋ - K＋ATPase of drought resistant variety in mitochondria and chloroplast was higher than thatof the first 4 days but 1ower in cytosol,which was still higher than that of the control,whereas the Na＋－K＋ATPase of the drought sensitive variety was lower than that of thecontrol.

关 键 词：荔枝 水分胁迫 ATPASE活性 质膜透性 抗旱性 

分 类 号：S667.101[农业科学—果树学]