外源NO对渗透胁迫下红三叶幼苗生长抑制及氧化损伤的缓解效应  

Alleviative Effects of Exogenous Nitric Oxide on Growth Inhibition and Its Oxidative Damage in Red Clover Seedlings under Osmotic Stress

作　　者：高景慧[1] 席雪丽[1] 刘记[1] 闫文[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《种子》2009年第8期24-28,共5页Seed

基　　金：教育部重点项目(105167)

摘　　要：25%的聚乙烯醇-6000(PEG-6000)渗透胁迫下,研究一氧化氮(NO)对红三叶(Trifolium pretenseL.cv.Altaswede)幼苗生长抑制及氧化损伤的缓解效应。结果表明:(1)0.05 mmol/L NO供体硝普钠(Sodium nitroprusside,SNP)可显著减轻渗透胁迫对幼苗生长的抑制,显著促进幼苗叶片超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和过氧化物酶(peroxidase,POD)的活性,降低丙二醛(malondialdehyde,MDA)含量,显著缓解渗透胁迫对红三叶造成生长抑制和氧化损伤作用;(2)高浓度(0.1 mmol/L)SNP处理对渗透胁迫的缓解效应不佳,甚至加重对植物的氧化损伤。Used 0.05 and 0. 1 mmol/L sodium nitroprussice （SNP）, an exogenous nitric oxide donor as NO supply body to study the effect on growth inhibition and its oxidative damage of red clover under osmotic stress produced by 25% PEG-6000. The results showed that,with its concentration 0.05 mmol/L sodium nitroprusside（SNP） ,an NO donor,specifically alleviate the inhibitory effect of seedlings growth and its oxidative damage under osmotic stress, plant height and root length increased prominently, protective enzyme activities of peroxidase（POD） ,superoxide dismutase （SOD） and catalase（CAT） of red clover also improved significantly. Moreover, the leaves of malondialdehyde （MDA） of red clover decreased remarkably. In particular, the effect of 0.05 mmol/L SNP was much more significant compared with 0.1 mmol/L SNP.

关 键 词：红三叶 一氧化氮(NO) 幼苗生长 抗氧化酶 渗透胁迫 

分 类 号：S311[农业科学—作物栽培与耕作技术]