原发性高血压患者GMP-140的研究——附62例报告  

Study on platelet alpha granule membrane protein-140 in the patients with essential hypertension

作　　者：刘兴德[1] 魏云鸿[1] 

机构地区：[1]贵阳医学院附属医院心血管科,550004

出　　处：《新医学》2009年第9期581-583,共3页Journal of New Medicine

基　　金：贵阳市科学技术计划项目(2006筑科农字27-5号)

摘　　要：目的:探讨原发性高血压(essential hypertension,EH)患者外周血中血小板α颗粒膜蛋白-140(plateletalpha granule membrane protein-140,GMP-140)水平及其单个核细胞中GMP-140基因的表达情况。方法:采用ELISA法测定62例EH患者(EH组)和30名健康体检者(对照组)外周血血清GMP-140含量,用逆转录-PCR法检测外周血单个核细胞中GMP-140mRNA的表达情况。结果:EH组外周血血清GMP-140为(190±70)μg/L,明显高于对照组的(122±50)μg/L(P<0.01);EH组单个核细胞GMP-140mRNA表达水平为0.58±0.17,与对照组0.26±0.11比较差异有统计学意义(P<0.01)。结论:EH患者存在GMP-140水平升高,外周血单个核细胞的GMP-140mRNA表达上调可能是EH患者外周血GMP-140含量增高的机制之一。Objective： To investigate the expression of platelet alpha granule membrane protein-140 （GMP-140） in peripheral blood and GMP-140 mRNA in peripheral blood mononuclear cell of the patients with essential hypertension （EH）. Methods： The serum GMP-140 was measured by enzyme linked immunosorbent assay （ELISA） in 62 patients with EH（EH group） and 30 healthy people （control group）, and GMP-140 mRNA of peripheral blood mononuclear cell expression was analyzed by reverse transcription polymerase chain reaction （RT- PCR）. Results： The serum GMP-140 was （190 ±70）μg/L in EH group, and was significantly higher than that of control group （122±50）μg/L （P〈0.01）. The level of mononuclear cellular GMP-140 mRNA was 0.58±0.17 in EH group, and was 0.26±0.11 in control group （P〈0.01）. Conclusion： The serum GMP-140 showed a significantly high level in the patient with EH, and up-regulating of mRNA GMP-140 may be one of the mecha-nisms of increasing GMP-140 in peripherial blood.

关 键 词：血小板Α颗粒膜蛋白-140 原发性高血压 信使核糖核酸 血小板 逆转录-聚合酶链反应 

分 类 号：R544.1[医药卫生—心血管疾病]