导数光谱法同时测定甲硝唑和维生素B_6的研究  

SIMULTANEOUS DETERMINATION OF METRONIDAZOLUM AND VITAMIN B6 BY THE DERIVATIVE SPECTROPHOTOMETRY

作　　者：张淑芳[1,2] 李怀娜[1,2] 张冬梅 周奇志[1,2] 

机构地区：[1]曲阜师范大学化学系 [2]山东威海市药品检验所

出　　处：《光谱学与光谱分析》1998年第4期488-491,共4页Spectroscopy and Spectral Analysis

摘　　要：本文建立了以０．１ｍｏｌ／ＬＨＣｌ为介质，采用三阶导数光谱法，同时测定甲硝唑和维生素Ｂ６的新方法。方法检测限为０．８１μｇ／ｍＬ甲硝唑和０．１６μｇ／ｍＬ维生素Ｂ６。二者测定的线性范围均为１．６～２２．４μｇ／ｍＬ，直接用于复方甲硝唑片剂中甲硝唑和维生素Ｂ６的测定。两种物质的测定变异系数分别小于２．２６和２．６０％，回收率分别为９３～１０２％和９９～１０１％。A new method for simultaneous determination of metronidazolum and vitamin B6 was established by the third derivative spectrophotometry in 0.1mol/L HCl solution.The detection limits were 0.81μg/mL for metronidazolum and 0.16μg/mL for vitamin B.6.The linear range of determination was 1.6～22.4μg/mL for both metronidazolum and vitamin B.6.This method has been applied to the determination of metronidazolum and vitamin B6 in tabellae metronidazoli compositae with satisfactory results.The coefficients of variation are below 2.26 and 2.65% and the recoveries are 93—102% and 99—101% for metronidazolum and vitamin B6,respectively.

关 键 词：导数光谱法 甲硝唑 维生素B6 复方甲硝唑 测定 

分 类 号：R978.69[医药卫生—药品]