隧道式拖线法对瘘管大鼠Ⅰ型和Ⅲ型胶原表达的影响  

Effect of Tunnel Thread-dragging Therapy on Collagen Type Ⅰ,Ⅲ Content in Rat with Fistula Wound Tissues

作　　者：王琛[1] 陆金根[1] 曹永清[1] 郭修田[1] 梁宏涛[1] 

机构地区：[1]上海中医药大学附属龙华医院肛肠科,上海200032

出　　处：《中国中西医结合外科杂志》2009年第4期428-432,共5页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：上海市卫生局青年基金项目(2007Y63);国家十一五科技支撑计划项目(2007BAI20B04)

摘　　要：目的：通过对体表瘘管大鼠模型隧道式拖线法治疗,研究在治疗不同时期Ⅰ型和Ⅲ型胶原表达量的变化。方法：采用免疫组化法在隧道式拖线法后第3、5、7、9、14 d和完全愈合时取大鼠局部组织进行Ⅰ型和Ⅲ型胶原表达量测定。结果：治疗组和对照组愈合时间分别为（16.25±1.96）d和（23.75±0.96）d,具有显著性差异。治疗组Ⅰ型胶原表达量在术后第9 d出现明显下降后逐级上升,在第14 d及愈合时治疗组高于对照组和空白组（P〈0.05）,而Ⅲ型胶原表达量在第9 d至第14 d上升,且高于对照组和空白组,具有显著性差异（P〈0.01）。结论：在隧道式拖线法后第9~14 d大鼠的Ⅰ/Ⅲ型胶原表达量比值升高,有助于创面愈合。Objective To evaluate the effects of tunnel thread - dragging through fistula method in rat with fistula. Methods Seventy - two male SD rats with fistula were randomly divided into study group （with the method of tunnel thread- dragging through the fistula）, control group （with the method of incision of the fistula） and the blank group （without any therapy） . The healing time and curative rate of fistula after treatment were examined. Changes of collagen type Ⅰ and Ⅲ contents in wound tissues were measured on the 3rd, 5th, 7th, 9th and 14th d and the cure day. Results The healing time of rat with fistula in the study group and the control group was 16.25 ± 1.96 d and 23.75 ± 0.96 d respectively, with significant differences between the two groups. The tunnel thread - dragging through fistula therapy could improve the collagen type Ⅰ and III contents after 14 days as compared to the control and blank groups, with significant differences. Conclusion Using tunnel thread - dragging therapy the ratio of collagen type Ⅰ/Ⅲ content can be increased after 9 - 14 days, as favorable indication of wound healing.

关 键 词：隧道式拖线法 瘘管大鼠 Ⅰ型胶原 Ⅲ型胶原 

分 类 号：R657.16[医药卫生—外科学] Q95-33[医药卫生—临床医学]