胶质细胞源性神经营养因子在慢传输型便秘大鼠结肠的表达及其对肠道传输功能的影响  

The expression of gllal cell line-derived neutrophic factor and its effect on colonic transit function in slow transit constipation rats

作　　者：李延玲[1] 吕宾[1] 范一宏[1] 

机构地区：[1]浙江中医药大学附属第一医院消化内科,杭州310006

出　　处：《中华内科杂志》2009年第9期752-755,共4页Chinese Journal of Internal Medicine

摘　　要：目的研究胶质细胞源性神经营养因子（GDNF）在慢传输型便秘（STC）大鼠结肠的分布、表达及外源性GDNF对肠道传输功能的影响。方法清洁级成年SD大鼠48只，雌雄各半，随机分为正常对照组和模型组，后者用大黄灌胃，共3个半月，建立STC的动物模型，前者用生理盐水灌胃。建模成功后正常对照组再分为正常组、GDNF组2个亚组，模型组再分为STC模型组、STC模型加GDNF组2个亚组。正常组和STC模型组经尾静脉注射生理盐水，GDNF组和STC模型加GDNF组经尾静脉注射外源性GDNF，连用1周。采用墨汁推进试验测定各组大鼠肠道传输功能，采用RT—PCR方法测定各组结肠GDNF的分布和表达。结果（1）各组的黑染肠管占肠管总长度的百分比分别为：正常组（74．44±2．19）％，STC模型组（60．00±5．62）％，STC模型加GDNF组（74．67±7．07）％，GDNF组（88．54±3．22）％，正常组与STC模型组、STC模型加GDNF组与STC模型组比较，差异有统计学意义（P值均〈0．01）。（2）结肠组织中GDNF mRNA的表达：STC模型组（1．38）与正常组（2．29）相比（P=0．01），STC模型组与STC模型加GDNF组（2．21）相比（P=0．017），GDNF mRNA相对表达水平差异有统计学意义；正常组与STC模型加GDNF组的差异无统计学意义。结论STC大鼠结肠GDNF的表达下调，在STC发病中起一定作用；STC模型组肠道传输明显减慢，外源性GDNF对肠道传输功能有明显促进作用。Objective To investigate the distribution and expression of glial cell line-derived neutrophic factor（GDNF） in colon of slow transit constipation （STC） rats and the effect of exogenous GDNF on colon transit. Methods Forty-eight SD rats were divided randomly into controlled group and model group. The models were established by gastric irrigation of rhubarb for 3. 5 months. The control group received normal saline. The models were successfully established and then the rats were divided randomly into four groups. They were a normal group, GDNF group, STC model group and a STC models plus GDNF group. Half of the rats in model and control group were administrated with exogenous GDNF intraveously for one week. The expression of GDNF and GDNF-mRNA in colon was detected with immunohistoehemistry and RT-PCR. The colon transmit speed was measured with Chinese ink driving test. Results The colon transmit of the STC model group, the normal group, the STC models plus GDNF group and GDNF group were （ 60. 00 ± 5.62 ） %, （ 74. 44 ± 2. 19 ） %, （ 74.67 ± 7.07 ） % and （ 88.54 ± 3.22 ） %. There was significant difference between the normal group and the STC model group（P 〈0. 01 ） as well as the STC models plus GDNF group and the STC model group （P 〈0. 01 ）. The expression of GDNF with reverse transeriptase PCR in the STC model group, the normal group and the STC models plus GDNF group was 1.38,2. 29 and 2. 21 respectively, with significant difference between the STC models and the normal group （P = 0. 01 ） and between the STC models and the STC models plus GDNF group （P = 0. 017 ）. However, there was no significant difference between the normal group and the STC models plus GDNF group （ P 〉 0. 05 ）.Conclusions The down regulation of GDNF in colon may play an important role in pathogenesis of STC Exogenous GDNF may improve the colon motor function by up-regulation of GDNF.

关 键 词：神经生长因子 便秘 肠道传输功能 

分 类 号：R686[医药卫生—骨科学]