SHP-2^(C459/S)突变体在三氧化二砷诱导的HEK293T细胞凋亡中的作用  

The role of SHP-2^(C459/S) mutant in apoptosis of HEK293T cell induced by arsenic trioxide

作　　者：董红岩[1] 司云凤[1] 孙东升[1] 祝晓春[1] 齐琦[1] 王果元[1] 金成艳[1] 王丽娜[1] 李全凤[1] 赵雅君[1] 田野[1] 徐长庆[1] 张力[1] 

机构地区：[1]哈尔滨医科大学病理生理学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2009年第4期313-315,共3页Journal of Harbin Medical University

基　　金：黑龙江省卫生厅资助项目(2005-36);哈尔滨医科大学留学归国人员科研启动基金资助

摘　　要：目的观察三氧化二砷(As2O3)对293T细胞凋亡和增殖的影响并探讨SHP-2在其中的作用。方法显性负性SHP-2突变体SHP-2C459/S与具有绿色荧光蛋白基因的载体pEGFP-C1共转染入HEK293T细胞,Ho-echst33258染色检测细胞凋亡,细胞计数及荧光分光光度法检测细胞增殖。结果SHP-2C459/S突变体能增强细胞凋亡和减轻增殖抑制。结论SHP-2可能参与到As2O3诱导的细胞凋亡和增殖抑制的信号转导通路中。Objective To investigate potential role （ As2O3 ）. Methods Dominant negative SHP-2^C459/S of SHP-2 in apoptosis induced by arsenic trioxide mutant was cotransfected with a vector harbouring green fluorescent protein gene pEGFP-C1 （as the marker gene） into HEK293T cells. Hoechst33258 staining were used for apoptosis assays, and cell counting and fluorescence spectrometry were used for proliferation assays. Results The introduction of SHP-2^C459/S mutaht increased the apoptosis and decreased the proliferation inhibition. Conclusion SHP-2 may involve in the pathways of apoptosis and prolifera- tion inhibition induced by As2O3.

关 键 词：SHP-2 三氧化二砷 凋亡 增殖 

分 类 号：R979.1[医药卫生—药品]