荧光探针法研究胶原蛋白的水溶液聚集状态  

A STUDY ON COLLAGEN ASSOCIATION STATE IN AQUEOUS SOLUTION USING FLUORESCENCE PROBE

作　　者：史向阳[1] 吴世康[1] 孙曹民[2] 

机构地区：[1]中国科学院感光化学研究所 [2]北京理工大学生物材料实验室

出　　处：《高分子学报》1998年第4期406-411,共6页Acta Polymerica Sinica

摘　　要：利用一种分子内电荷转移化合物３甲氧基４’Ｎ，Ｎ二甲氨基黄酮（ＤＭＭＦ）作为荧光探针，研究了胶原蛋白在水溶液中的聚集状态．研究结果发现，当胶原蛋白浓度增大到０５ｍｇ／ｍＬ以上时，ＤＭＭＦ在４６５ｎｍ处的荧光强度有一突跃；继续增大其浓度，荧光强度越来越大．证明胶原蛋白在水溶液中随浓度增大有明显的聚集行为．利用荧光偏振法测量该体系内ＤＭＭＦ的荧光偏振特性，发现当胶原蛋白浓度增大到０５ｍｇ／ｍＬ以上时，体系具有较高的微粘度，进一步证实了胶原蛋白在水溶液中的聚集特性．A kind of intramolecular charge transfer compound——3 methoxy 4' N,N dimethylamino flavone derivateves(DMMF) was used to detect collagen association in aqueous solution.Results showed that,when collagen concentration was increased to 05mg/mL or above,the fluorescence intensity of DMMF at 465nm increased dramatically.This indicated that collagen can associate in aqueous solution.A fluorescence polarization study on the same collagen solution also showed that the fluorescence polarization of DMMF rose rapidly when collagen concentration was increased to 05mg/mL or above,which proved that the system had high micro viscosity.This results also indicated that the aggregation behavior existed in the aqueous solution of collagen.

关 键 词：荧光探针 胶原蛋白 荧光偏振 聚集状态 水溶液 

分 类 号：Q510.3[生物学—生物化学]