大麻素受体1参与重复电针预处理诱导的大鼠脑缺血耐受  

Involvement of cannabinoid receptor 1 in ischemic tolerance induced by pretreatment with repeated electroacupuncture in rats

作　　者：胡博[1] 王强[1] 杜鹃[1] 朱萧玲[1] 侯丽宏[1] 朱正华[1] 侯立朝[1] 董海龙[1] 熊利泽[1] 陈绍洋[1] 

机构地区：[1]第四军医大学西京医院麻醉科,陕西西安710033

出　　处：《第四军医大学学报》2009年第16期1476-1479,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30572421;30873326)

摘　　要：目的:探讨大麻素受体1(CB1受体)是否参与重复电针预处理诱导的大鼠脑缺血耐受.方法:48只健康雄性SD大鼠(280～320g),随机分为6组(n=8):假手术组(Sham),戊巴比妥钠对照组(Con),电针预处理组(EA),溶剂-电针预处理组(v-EA),CB1受体拮抗剂组(AM),CB1受体拮抗剂-电针预处理组(AM-EA).除Sham组外其余各组动物每日均腹腔注射(ip)戊巴比妥钠40mg/kg麻醉,连续5d.Sham组仅行假手术;Con组仅缺血再灌注;EA组接受电针刺激百会穴30min/d,连续5d;AM组每日腹腔注射AM2511mg/kg,连续5d;v-EA组和AM-EA组每日在电针预处理前30min分别给予溶剂(二甲基亚砜∶Tween-80∶生理盐水=1∶1∶8)3mL/kg(ip)和AM2511mg/kg(ip),连续5d.除Sham组外其余各组均在最后一次预处理24h后行颈内动脉尼龙线线栓法致大脑中动脉栓塞(120min)模型,观察再灌注后24h时神经功能评分(NFS),并取大脑行2,3,5-氯化三苯四唑(TTC)染色以计算脑梗死容积百分比.结果:Sham组NFS正常且TTC染色未见脑梗死灶;再灌注24h后EA组和v-EA组NFS明显高于Con组、AM组和AM-EA组(P<0.05);EA组和v-EA组再灌注24h脑梗死容积百分比分别为(31.4±8.3)%和(27.2±9.3)%,明显小于Con组、AM组和AM-EA组(P<0.05);而AM组、AM-EA组与Con组之间NFS和脑梗死容积百分比均无统计学意义.结论:重复电针刺激大鼠百会穴预处理诱导的脑缺血耐受可能通过CB1受体介导.AIM： To explore whether cannabinoid receptor 1 （ CB1 ） participates in the ischemic tolerance induced by pretreatment with repeated electroacupuncture. METHODS： Forty-eight male SD rats were randomly assigned to 6 groups （ n = 8 in each group）： Sham group, control （Con） group, electroaeupuncture （EA） group, vehicle plus electroaeupuncture （v-EA） group, CB1 receptor antagonist （AM） group and CB1 receptor antagonist plus eleetroacupuncture （AM-EA） group. All the animals were anesthetized with intraperitoneal injection of 40 mg/kg pentobarbi- tal sodium once a day for 5 consecutive days except those in Sham group. Rats in Sham group only received sham operation, rats in Con group were only subjected to ischemia and reperfusion, rats in EA group received electroacupuncture at the Baihui acupoint for 30 min a day for 5 consecutive days, rats in AM group received AM251 1 mg/kg （ip） once a day for 5 consecutive days, and rats in v-EA group and AM-EA group received electroacupuncture at the Baihui acupoint for 30 rain and were respectively injected with vehicle （ DMSO： Tween-80： saline = 1 ： 1 ： 8 ） 3 mL/kg and AM251 1 mg,/kg （ip） 30 min prior to the onset of clectroacupuncture once a day for 5 consecutive days. Twenty-four hours after last treatment, all groups except Sham group received the middle cerebral artery occlusion. The neurological function scores （NFS） of all the rats were evaluated at 24 h after reperfusion. The brain infarct volume, as a percentage of volume at normal cerebral hemisphere, was then assessed by 2, 3, 5 triphenyltetrazolium chloride （TFC） staining after the evaluation of NFS. RESULTS： In Sham group, no neurological deficits and infarct volume were observed. The NFS in EA group and v-EA group were significantly higher than that in Con group, AM group and AM-EA group （P 〈 0.05）. The infarct volume percentage in EA group and v-EA group was （31.4 ±8.3）% and （27.2 ±9.3）%, which was significantly smaller than that in Con

关 键 词：电针 预处理 再灌注损伤 受体 大麻酚 大鼠 

分 类 号：R651.15[医药卫生—外科学]