超高效液相色谱法测定天然原料中的香豆素含量  

Determination of Coumarin in Natural Raw Materials with UPLC

作　　者：刘正聪[1,2] 陆舍铭 王海利[1,3] 招云芳[1,2] 李中昌 王迪 缪明明 

机构地区：[1]红塔烟草集团有限责任公司技术中心,玉溪653100 [2]云南大学化学科学与工程学院,昆明650091 [3]云南民族大学化学与生物技术学院,昆明650031

出　　处：《香料香精化妆品》2009年第4期27-29,共3页Flavour Fragrance Cosmetics

摘　　要：建立了超高效液相色谱法测定天然原料中香豆素的方法。以无水乙醇作溶剂,超声萃取5min,萃取液经0.22μm滤膜过滤后,用配置TUV检测器的超高效液相色谱仪于274nm波长下对6种天然原料中香豆素进行测定,结果表明:香豆素在0.5～8.0μg间呈线性关系(R=0.9999)。相对标准偏差为1.06%,回收率在97.3%～99.0%之间,检测限为11.02ng/g。A method of determination of coumarin in natural raw materials with UPLC was established. The samples were extracted with ethanol under ultrasonic for 5 min, filtered with 0.22 /Lm filter membrane, then detected by UPLC with TUV at 274 nm, respectively. The results showed： the linear range was 0. 5 ~8. 0 μg （R = 0. 9999） for coumarin, the RSD was 1.06% ,the recovery was from 97.3% to 99. 0%, and the limit of detection was 11.02 ng/g.

关 键 词：超高效液相色谱 天然原料 香豆素 

分 类 号：TQ651[化学工程—精细化工]