甲氰咪呱对K562细胞诱导脐血T细胞TCR Vβ亚家族克隆性的影响  

Effect of cimetidine on clonal expansion of TCR Vβ subfamily of T cells in cord blood stimulated by K562 cells

作　　者：田红霞[1] 林晨[1] 陈少华[2] 杨力建[2] 江振友[1] 高珂[1] 郜世隽[1] 李扬秋[2] 

机构地区：[1]暨南大学医学院微生物学与免疫学教研室,广州510632 [2]暨南大学医学院血液病研究所,广州510632

出　　处：《第三军医大学学报》2009年第17期1637-1640,共4页Journal of Third Military Medical University

基　　金：国家高技术研究发展计划(863计划)(2006AA02Z114);广东省自然科学基金(06025169);暨南大学病理生理重点实验室开放课题基金(2006)~~

摘　　要：目的研究甲氰咪呱(cimetidine)对K562细胞体外诱导脐血T细胞TCR Vβ亚家族克隆性的影响。方法体外分别将甲氰咪呱、K562细胞、甲氰咪呱+K562细胞与正常人的脐带血单个核细胞(mononuclear cells,MNC)混合培养2周,检测诱导增殖T细胞的特异杀伤活性,同时利用RT-PCR基因扫描技术分析培养后T细胞的TCR Vβ亚家族谱系的选择性利用和克隆性增殖情况。结果经甲氰咪呱、K562细胞、甲氰咪呱+K562细胞诱导培养2周后,各组均不同程度诱导细胞增殖,其中甲氰咪呱联合K562细胞诱导组的T细胞对K562细胞的特异性杀伤作用显著增强(P<0.01)。3组均呈现不同程度的T细胞TCR Vβ亚家族选择性利用,均有TCR Vβ21亚家族呈克隆性增殖。结论甲氰咪呱可协助增强bcr-abl抗原刺激的特异性抗CML T细胞作用。Objective To investigate the effect of cimetidine on the clonal expansion of TCR Vβ subfamily of T cells in cord blood after the stimulation by K562 cells in vitro. Methods Cimetidine （ 1 × 10^-5mol/L） or K562 cells （1 × 10^6/ml） or both of them were respectively cultured with mononuclear cells （MNC） isolated from normal human cord blood for 2 weeks. After the induction, specific cytotoxicity of the proliferated T cells were detected with K562 cells as the target cells. The selective usages and clonal expansion of TCR Vβ subfamily of T cells were analyzed by RT-PCR and genescan technique. Results After induction for 2 weeks, the 3 groups showed the increased cell proliferation, in which specific eytotoxicity of T cells induced by both cimetidine and K562 cells against K562 cells was enhanced significantly compared with the other 2 groups （P 〈 0. 01 ）. Differental selective usages of T cells TCR Vβ subfamily and TCR Vβ21 clonal proliferation were found significantly in every group. Conclusion Cimetidine enhances the specific cytotoxicity of T cells stimulated by chronic myelogenous leukemia L antigen

关 键 词：甲氰咪呱 T细胞受体VΒ基因 慢性粒细胞白血病 脐血 

分 类 号：R392.12[医药卫生—免疫学] R733.721[医药卫生—基础医学]