饲喂雌鼠大豆异黄酮对后代雌性生殖性状的影响  

Effect of Feeding Female Mice with Soy Isoflavones on Some ReproductivePhysiological Characteristics of their Female Offspring

作　　者：尹艳云[1] 刘京威[1] 鲍文玉[1] 秦国嵩[1] 刘云海[1] 王晓霞[1] 倪和民[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《中国实验动物学报》2009年第4期299-302,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：十一五国家支撑计划二级子项目(编号:2008BADB2B04-4);北京市科委资助项目(D08060500060802)

摘　　要：目的研究雌性小鼠饲喂异黄酮后对其后代雌鼠生殖性状的影响。方法分别在雌鼠从断奶到妊娠前和妊娠期间两个不同阶段的日粮中添加不同剂量的大豆异黄酮(0、50、400 mg/kg)。记录F1代的出生窝产仔数、出生窝重、雌鼠的阴道最早开张时间和最早见栓时间,以及后代雌鼠性成熟期和体成熟期生殖器官重量、血清雌激素的含量。结果雌鼠妊娠期饲喂含50、400 mg/kg大豆异黄酮的饲料后F1代的窝产仔数显著高于对照组(P<0.05);雌鼠妊娠期采食400 mg/kg大豆异黄酮饲料,其后代雌鼠初次配种时间明显延迟(P<0.05);45、65日龄体重显著低于对照组,65日龄子宫重显著低于对照组,卵巢重显著高于对照组;血清中雌激素含量显著低于对照组(P<0.05),妊娠期间采食50 mg/kg大豆异黄酮饲料,其F1代雌鼠仅窝产仔数和45日龄时血清中雌激素含量受到影响;而雌鼠从断奶到怀孕期间饲喂含大豆异黄酮饲料的F1代在各项指标与对照组均差异无显著性。结论雌鼠妊娠期间饲喂含400 mg/kg大豆异黄酮的饲料会显著影响其雌性后代的初情期、生殖器官发育、血清雌激素含量等生殖生理性状,而在非妊娠期饲喂含大豆异黄酮饲料对F1代无显著影响。Objective In this experiment,it is aimed to investigate the effect of feeding female mice with isoflavones on some physiological characteristics of their female offspring.Methods The ICR female mice were divided into the control（0 mg/kg）,low soy isoflavone level（50 mg/kg） and high soy isoflavone level（400 mg/kg） diet groups separately at the gestation period and prior to conception.In addition,the state of vagina opening and vagina plug of offspring after treatment with soy isoflavones,the concentration of estrogen in their serum on Day 45 and Day 65 as well as their body,uterus and ovary weight,litter size and their birth weight,were also recorded and analyzed separately.Results The litter size of those female mice feeding with 50 mg/kg or 400 mg/kg soy isoflavones,was significantly higher than that in the control group（P0.05）.The state of vagina opening and vagina plug of the offspring whose mothers feeding with 400 mg/kg soy isoflavones during their gestation period,was significantly delayed than that of control one（P0.05）.Their body weight on Day45 and Day65,uterus weight on Day 65,the concentration of serum estradiol on Day 65 were all significantly lower than those in control group respectively,but the ovary weight on Day 65 was significantly higher than it（P0.05）.The female F1 generation＇s litter size and concentration of serum estradiol on Day 45 were obviously affected due to their mothers intaking 50 mg/kg soy isoflavones during their gestation period,but there were no significant differences among those different groups of female F1 generation whose mothers intaking soy isoflavones between their weaning and pre-gestation period（P0.05）.Conclusion Collectively,it can be concluded that the female F1 generation＇s some reproductive characteristics such as the initial oestrus,development of reproductive organs and concentration of serum estradiol were affected significantly,due to their mothers intaking 400 mg/kg soy isoflavones during their gestation period,but were not affec

关 键 词：ICR雌鼠 大豆异黄酮 雌性后代 生殖性状 

分 类 号：R241[医药卫生—中医诊断学]