Local Immune Regulatory Effects of Bangdeyun on the Endometrium of Mice with Embryo Implantation Dysfunction during the Implantation Time  

作　　者：吴云霞 郑翠红 胡琳丽 李靖 

机构地区：[1]Hubei Key Laboratory of Natural Pharmacochemistry and Resources Evaluation, Department of Traditional Chinese Herbs, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology [2]Institute of Integrated Traditional Chinese Medicine and Western Medicine,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology [3]Center of Reproductive Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology [4]Department of Emergency, Hubei General Hospital of Chinese People’s Armed Police Forces

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2009年第3期372-376,共5页华中科技大学学报（医学英德文版）

基　　金：supported by a grant from National Natural Science Foundation of China (No. 30801538)

摘　　要：This study examined the effects of Bangdeyun on the expressions of nuclear factor-kappaB （NF-κB）, interferon-gamma （IFN-y） and interleukin-10 （IL-10） in the endometrium of mice with embryo implantation dysfunction （EID） during the implantation time （namely on pregnancy day 5, 6, 7 and 8） and explored the local immune regulatory effects of Bangdeyun. The gestational mice were randomly divided into normal group, model group and Bangdeyun-treated group. EID models of mice were established by using indomethacin. The endometrial expression of NF-κB was detected by immunohistochemistry and Western blotting. IFN-γ and IL-10 were measured by enzyme-linked immunosorbent assay （ELISA）. The results showed that in the normal group, NF-κB and IFN-γ were weakly expressed and IL-10 was strongly expressed in the endometrium during the whole implantation period. In the model group, the expressions of NF-κB and IFN-T were increased on pregnancy day 5, 6 and 7, and IL-10 expression decreased during the whole implantation time when compared with those in the normal group （P〈0.01 for all）. In the Bangdeyun-treated group, little amount of NF-κB and IFN-γ was expressed and IL-10 expression was strong, much the way they were expressed in the normal group （P〉0.05）. The expressions of NF-κB and IFN-T were much lower in the Bangdeyun-treated group than those in the model group on pregnancy day 5, 6 and 7 （P〈0.01 for all）, while the expression of IL-10 was much higher than in the model group during the whole implantation time （P〈0.01）. It was suggested Bangderun may favor a shift from Thl- to Th2-type immune response, therefore inhibiting the maternal immune rejection, inducing the immune tolerance and improving the fetal implantation.This study examined the effects of Bangdeyun on the expressions of nuclear factor-kappaB （NF-κB）, interferon-gamma （IFN-y） and interleukin-10 （IL-10） in the endometrium of mice with embryo implantation dysfunction （EID） during the implantation time （namely on pregnancy day 5, 6, 7 and 8） and explored the local immune regulatory effects of Bangdeyun. The gestational mice were randomly divided into normal group, model group and Bangdeyun-treated group. EID models of mice were established by using indomethacin. The endometrial expression of NF-κB was detected by immunohistochemistry and Western blotting. IFN-γ and IL-10 were measured by enzyme-linked immunosorbent assay （ELISA）. The results showed that in the normal group, NF-κB and IFN-γ were weakly expressed and IL-10 was strongly expressed in the endometrium during the whole implantation period. In the model group, the expressions of NF-κB and IFN-T were increased on pregnancy day 5, 6 and 7, and IL-10 expression decreased during the whole implantation time when compared with those in the normal group （P〈0.01 for all）. In the Bangdeyun-treated group, little amount of NF-κB and IFN-γ was expressed and IL-10 expression was strong, much the way they were expressed in the normal group （P〉0.05）. The expressions of NF-κB and IFN-T were much lower in the Bangdeyun-treated group than those in the model group on pregnancy day 5, 6 and 7 （P〈0.01 for all）, while the expression of IL-10 was much higher than in the model group during the whole implantation time （P〈0.01）. It was suggested Bangderun may favor a shift from Thl- to Th2-type immune response, therefore inhibiting the maternal immune rejection, inducing the immune tolerance and improving the fetal implantation.

关 键 词：Bangdeyun NF-κB INDOMETHACIN embryo implantation dysfunction maternal cytokines 

分 类 号：R714[医药卫生—妇产科学]