微乳液组成对毛细管微乳液电动色谱的影响  

Effects of Microemulsion Composition on Microemulsion Electrokinetic Capillary Chromatography

作　　者：吕建德[1] 傅小芸[1] 徐秀珠[1] 

机构地区：[1]浙江大学应用化学研究所

出　　处：《高等学校化学学报》1998年第8期1219-1222,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：研究了一组电中性芳香族化合物的毛细管微乳液电动色谱分离，考察分离介质微乳液的组成对电动色谱保留时间的影响．实验结果表明，各芳香化合物的保留时间均随表面活性剂浓度增加而增长；内相和助表面活性剂对有、无亲水基团芳香化合物的保留时间有不同的影响规律．选用微乳液组成为８０ｍｍｏｌ／Ｌ正庚烷－１２０ｍｍｏｌ／ＬＳＤＳ－９００ｍｍｏｌ／Ｌ正丁醇－１０ｍｍｏｌ／Ｌ硼砂，样品在１４ｍｉｎ内达到完全分离，理论板数为３×１０５～９×１０５／ｍ．A set of wide polar aromatic analytes was separated by microemulsion electrokinetic capillary chromatography(MEEKC). The effects of microemulsion compositions, including the core phases( n hexane, n heptane, n octane) alkyl chain length, the concentrations of core phase heptane, surfactant sodium dodecyl sulfate and co surfactant n butanol, on electrokinetic separation were studied. The results showed that the surfactant had the similar effects on the retention time of all analytes. And both core phase and co surfactant had different effects on analytes with hydrophilic functional groups and without any hydrophilic functional group. The separation mechanism of microemulsion electrokinetic chromatography was discussed. Using microemulsion consisting of 80 mmol/L n heptane 120 mmol/L SDS 900 mmol/L n butanol 10 mmol/L borate, the complete separation was obtained within 14 minutes. The numbers of theoretical plate ranged 3×10 5 -9×10 5 per meter.

关 键 词：毛细管电动色谱 微乳液 芳香族 MEEKC 保留时间 

分 类 号：O657.7[理学—分析化学] O625[理学—化学]