北京及周边地区乳牛乳腺炎的调查与病原菌分离鉴定  

Investigation of cow mastitis and the isolation and identification of its pathogenic bacteria in Beijing and its surrounding areas

作　　者：杨秀玉[1] 莫云[2] 冯华兵[3] 葛冰[2] 黄建茹[2] 丛日刚[2] 臧丽 刘澜[2] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]北京大北农集团动物保健技术研究中心,北京101407 [3]北京市农业局,北京100029 [4]北京市延庆县畜牧兽医总站,北京102100

出　　处：《中国兽医科学》2009年第8期743-745,共3页Chinese Veterinary Science

基　　金：北京市海淀区科技项目(k2008197)

摘　　要：为了解乳牛乳腺炎的发病原因,对北京及其周边地区4个奶牛场的乳牛乳腺炎的发病情况进行了临床调查、诊断和检测,采集患临床型乳腺炎和隐性乳腺炎乳牛的乳样;用接种法对采集乳样进行培养,对可疑菌落进行分离、接种、染色、镜检鉴定。结果显示,从采集的266份乳样中分离到7种434株病原菌,其中葡萄球菌150株,链球菌204株,革兰阴性杆菌80株。证实,北京及其周边地区乳牛乳腺炎的主要病原菌为葡萄球菌和链球菌,且细菌混合感染引起的乳牛乳腺炎有上升趋势。To understand the incident reasons of cow mastitis,266 milk samples collected from cow with clinical and recessive mastitis on four cattle farms in Beijing and its surrounding areas were examined through clinic investigation,diagnosis,detection,and cultivation.Suspicious colonies were isolated,inoculated,stained,and identified by microscopy.434 strains of pathogenic bacteria involved in 7 species of bacteria were isolated and identified from the milk samples,of which 150 strains were Staphylococcus,204 strains were Streptococcus,and 80 strains were Gram-negative bacilli.Staphylococcus and Streptococcus were the main pathogenic bacteria causing cow mastitis and cow mastitis caused by mixed bacteria infection were on upward trend in Beijing and its surrounding areas.

关 键 词：北京 乳牛 乳腺炎 病原菌 分离鉴定 

分 类 号：S857.26[农业科学—临床兽医学]