牛性别决定新基因Fgf9的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of Fgf9,a Novel Gene Related to Sex Determination in Cow

作　　者：廖冰[1] 吴宁[1,2] 韩凤桐[3] 林秀坤[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]中国科学院海洋研究所,青岛266071 [3]东北农业大学,哈尔滨150030

出　　处：《中国生物工程杂志》2009年第8期45-50,共6页China Biotechnology

基　　金：国家自然科学基金资助项目(30671501)

摘　　要：Fgf9基因是脊椎动物性别决定中重要的信号因子,它在睾丸发育过程中参与Sertoli细胞的增殖和睾丸索的形成。基于表达序列标签(expressed sequence tags,ESTs)克隆原理,采用序列拼接和RT-PCR方法获得了荷斯坦奶牛Fgf9基因的cDNA序列,并对其组织表达特征进行分析。利用生物信息学方法对Fgf9基因序列和蛋白结构进行分析。结果显示:Fgf9基因定位于牛12号染色体上,cDNA全长为697bp,开放阅读框为627bp,编码208个氨基酸,分子量23.38245kDa,等电点7.0600。RT-PCR证实该开放阅读框正确,在牛的各组织中均有表达,且与牛其他cDNA无同源性,获得GenBank登陆号为:EU693028。功能结构分析显示Fgf9蛋白具有典型的FGF家族保守结构域,包括受体相互作用位点和肝素结合位点。信号肽预测显示牛Fgf9蛋白可能不存在信号肽序列。Fgf9 is and the cord formation tags, sequence splicing the most important signal factor implicated in mediating the proliferation of Sertoli cells in testis morphogenesis of vertebrate. With the cloning principle of expressed sequence and RT-PCR were applied in the cloning of novel gene from Holstein cow and analyzing its expression pattern in various cow tissues. The bioinformatics analysis on the sequence and protein results of was conducted. Results indicated that Fgf9 was located 697bp. The open reading frame is 627bp in length and encodes at cow 12 chromosome, with full-sequence of a deduced amino acid sequence of 208 residues. The molecular weight of Fgf9 protein is 23. 38245kDa, and its pI is 7. 0600. RT-PCR demonstrated the correctness of its ORF was expressed in each tissue. No obvious homology with other cow cDNA was found for Fgf9. The GenBank accession number EU693028 was achieved. Function and structural analysis indicated that Fgf9 has a typical FGFs conserved domains, receptor interaction site and heparin binding site. It was predicted that Fgf9 has no signal peptide.

关 键 词：Fej9 性别决定 基因克隆 生物信息学 

分 类 号：Q811.4[生物学—生物工程] S823.91[农业科学—畜牧学]