直接重整细胞核程序的诱导性多能干细胞研究进展  

Looming of the Era for the Induced Pluripotent Stem Cells by Direct Nuclear Reprogram

作　　者：牟奕[1] 孙激[1] 

机构地区：[1]四川大学华西第二医院,成都610041

出　　处：《中国生物工程杂志》2009年第8期124-128,共5页China Biotechnology

摘　　要：诱导性多能干细胞(induced pluripotent stem cells,iPS)是分化细胞在外源性因子作用下,经直接细胞核程序重整而重新获得分化潜能的干细胞,具有很重要的应用前景。介绍了iPS诱导方法从转录因子、RNA结合蛋白、小分子化合物、到信号传导通路的发展过程,以及在提高生物安全性方面的改进。iPS的生成在细胞学上表现为渐进的、时间依赖的过程,同细胞的分化状态密切相关;然而,iPS同胚胎干细胞表遗传特征并非完全相同。iPS的进展结合基因治疗和细胞治疗的成果已应用到动物疾病模型的治疗。Induced pluripotent stem cells （iPS） are derived from the differentiated somatic cells that regain the differentiation capabilities by the direct nuclear reprogramming with the exogenous factors. The methods for iPS induction evolved from transcription factors to RNA binding proteins, small molecules, and extraeellular signals with the efforts in improving biosafty. The cellular and epigenetic characterization for iPS generation is a progressive and time-dependent process, which is tightly associated with the cellular differentiation status. However, epigenetics of iPS is not same as the embryonic stem cells. In combination with gene therapy and cellular transplantation therapy, the achievements of iPS had been applied in animal disease model treatment.

关 键 词：诱导性多能干细胞 直接程序重整 转录调控 细胞治疗 

分 类 号：Q291[生物学—细胞生物学]