氟比洛芬酯对大鼠局灶性脑缺血-再灌注损伤的保护作用  

Protective effects of flurbiprofen axetil preconditioning on focal cerebral ischemical-reperfusion injury in rats

作　　者：彭娜[1] 鲁瑶[1] 师晓琴[1] 董辉[1] 侯立朝[1] 汪晨[1] 

机构地区：[1]第四军医大学西京医院麻醉科全军临床麻醉中心,西安市710032

出　　处：《临床麻醉学杂志》2009年第8期704-706,共3页Journal of Clinical Anesthesiology

摘　　要：目的探讨氟比洛芬酯(FA)预处理(PC)对局灶性脑缺血-再灌注损伤保护作用。方法(1)雄性SD大鼠56只,随机均分为七组:缺血-再灌注组(I-R组)、FA5mg/kg组(FA5组)、FA10mg/kg组(FA10组)、FA20mg/kg组(FA20组)、FA40mg/kg组(FA40组)、脂微球组(Lip组)和假手术组(Sham组)。脑缺血前6h分别静注相应剂量FA及其脂微球载体,制作大脑中动脉阻塞(MCAO)模型,观察脑缺血-再灌注后24hGarcia法神经行为学评分(NBS),并行TTC染色计算脑梗死容积百分比(BIVP)。(2)雄性SD大鼠48只,随机均分为六组:I-R组、PC-6h组、PC-12h组、PC-24h组、PC-48h组和PC-72h组,分别于脑缺血前6、12、24、48及72h给予后五组20mg/kg选择剂量FA行单次预处理,制作MCAO模型,观察各组脑缺血-再灌注后24hNBS及BIVP变化。结果(1)FA5、FA10和FA20组不仅其脑缺血-再灌注后24hNBS均明显高于I-R组(P<0.05),且BIVP也较I-R组明显缩小(P<0.05)。(2)脑缺血前6、12、24及48h单次给予选择剂量20mg/kgFA进行预处理,其余各组脑缺血-再灌注后24hNBS均较I-R组有明显改善(P<0.05),且BIVP明显缩小(P<0.05)。结论FA单次预处理可明显减轻大鼠局灶性脑缺血-再灌注损伤,且预处理脑保护效应时间窗长达48h。Objective To investigate the protective effects of flurbiprofen axetil （FA） preconditioning（PC） on focal cerebral isehemical-reperfusion（I-R） injury in rats. Methods （1） Fifty-six male SD rats were randomly divided into 7 groups of I-R, FA 5 mg/kg, FA 10 mg/kg, FA 20 mg/kg, FA 40 mg/kg, Lip and Sham with 8 rats each. The dose of FA or lipo-microballoons solvent （the vehicle for FA）was intravenously administered to the corresponding groups at 6 h before the establishment of cerebral I-R model by middle cerebral artery occlusion（MCAO） for 120 mirL The neurological behavior score（NBS）was measured by Garcia scoring at 24 h after I-R. The brain infarct volume percentage（BIVP）was then assessed after 1% TTC staining following the last neurological outcome evaluation. （2）Fourty-eight male SD rats were randomly divided into 6 groups of I-R, PC-6 h, PC-12 h,PC-24 h,PC-48 h and PC-72 h with 8 rats each and FA 20 mg/kg was given at 6,12,24,48 or 72 h before cerebral ischemia, respectively, in the last five groups. The I-R model was established and NBS and BIVP were recorded as mentioned above. Results （1）The NBS in group FA 5 mg/kg, FA 10 mg/kg and FA 20 mg/kg at 24 h after I-R was not only greatly higher than that in group I-R（P %0. 05） ,but the BIVP was reduced more than that in group I-R（P〈0. 05）. （2） NBS and BIVP were significantly improved when FA 20 mg/kg was given at 6, 12, 24 or 48 h before cerebral ischemia compared with those in group I-R （P 〈 0.05 ）. Conclusion FA preconditioning may significantly reduce the focal cerebral I-R injury induced by MCAO in rats. The therapeutic time window of FA neuoprotection may be over 48 hours.

关 键 词：氟比洛芬酯 脑缺血-再灌注损伤 神经保护作用 

分 类 号：R614[医药卫生—麻醉学]