未成熟树突状细胞联合雷帕霉素诱导小鼠皮肤移植免疫耐受  

Induction of tolerance to mouse skin allograft by donor bone marrow-derived immature dendritic cells combined with rapamycin

作　　者：余思[1] 付必莽[2] 何晓顺[2] 胡安斌[2] 马毅[2] 黄洁夫[2] 

机构地区：[1]中山大学附属第六医院胃肠外科,广州510655 [2]中山大学附属第一医院器官移植科

出　　处：《中华实验外科杂志》2009年第9期1127-1129,共3页Chinese Journal of Experimental Surgery

基　　金：广东省自然科学基金资助项目（06104600）;China Medical Boardin New York（06837）.

摘　　要：目的观察供者骨髓来源的未成熟树突状细胞联合雷帕霉素在诱导小鼠同种异基因皮肤移植免疫耐受中的协同作用。方法健康雄性C57BL/6小鼠和BALB／C小鼠分别为供者、受者，建立同种异基因皮肤移植模型。对照组术前术后未给予任何免疫干预措施；雷帕霉素组术后第0天至第6天给予雷帕霉素灌胃；未成熟树突状细胞组将供者骨髓来源的未成熟树突状细胞于术前第7天经尾静脉输注给受者；联合组将供者骨髓来源的未成熟树突状细胞于术前第7天经尾静脉输注给受者，并在术后第0天至第6天给予雷帕霉素灌胃。结果对照组、雷帕霉素组、未成熟树突状细胞组、联合组的皮肤移植物存活时间分别为（6．9±1．9）、（10．3±3．0）、（17．0±3．4）、（20．8±3．6）d。方差分析提示组间差异有统计学意义（P〈0．05）；S-N—K检验提示各组之间的差异均有统计学意义（P〈0．05）。结论供者骨髓来源未成熟树突状细胞可诱导小鼠皮肤移植免疫耐受；联合使用雷帕霉素可延长免疫耐受的持续时间。Objective To investigate the synergic effects of donor bone marrow-derived immature dendritic cells combined with rapamycin on induction of tolerance to skin allograft in mice. Methods An allogenic skin transplantation model was established with the donors C57BL/6 mice and the recipients BALB/C mice. Recipients were divided into 4 groups ： control group （ no administration before or after skin transplantation） , rapamycin group （receiving rapamycin 1 mg/kg^-1·d^-1 by garage for consecutive 7 days after skin transplantation） ,immature DC group （receiving an injection of donor bone marrow-derived immature DC via tail vein before skin transplantation）, combined group （receiving an injection of donor bone marrow-derived immature DC via tail vein before transplantation and rapamycin 1 mg/kg^-1·d^-1 by garage for consecutive 7 days after transplantation）. The survival time of skin allograft was observed. Results The survival time of skin allograft in the groups of control, rapamycin, immature DC, and immature DC ± rapamyein was （6.9 ± 1.9 ）, （ 10.3 ± 3.0）, （ 17.0 ± 3.4 ）, and （ 20.8 ± 3.6 ） days respectively. Statistical analysis showed that there was significant difference among groups （ P 〈 0.05 ） ; Furthermore, S-N-K test showed that there was significant difference between every two groups. Conclusion There were synergic effects for donor bone marrow-derived immature dendritic cells and rapamycin in induction of tolerance to skin allograft in mice.

关 键 词：树突状细胞 雷帕霉素 免疫耐受 皮肤移植 

分 类 号：R392[医药卫生—免疫学]