精氨酸-甘氨酸-天冬氨酸肽联合环氧氯丙烷改良去细胞猪瓣研究  

RGD peptides and epoxy chloropropane modify decellular porcine valve scaffold

作　　者：顾春虎 王云雅 魏旭峰 董小超 易定华 孙国成 

出　　处：《中华实验外科杂志》2009年第9期1188-1189,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30600137）;国家“863”计划重点研究计划资助项目（2006AA02A138）

摘　　要：目的观察精氨酸-甘氨酸-天冬氨酸（RGD）肽联合环氧氯丙烷（EC）对去细胞猪主动脉瓣构建组织工程心脏瓣膜（TEHV）的影响。方法应用化学交联剂EC，使酪氨酸-甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸．脯氨酸（YGRGDSP）肽与去细胞猪主动脉瓣支架稳定结合同时交联固定支架内胶原纤维，然后种植大鼠骨髓干细胞以初步构建TEHV。实验分为RGD组，去细胞组和RGD＋EC处理组。结果RGD＋EC组断裂强度（785．8±13．2）g／mm^2和细胞黏附16h贴壁率（0．72±0．16），优于RGD组和去细胞组断裂强度[（621．2±7．9）、（628．4±5．3）g／mm^2，P〈0．05]和贴擘率[（0．41±0．10），（0．47±0．14），P〈0．05]；光镜和电镜观察RGD＋EC组种植效果优于RGD组和单纯去细胞组。结论RGD肽联合EC改良去细胞瓣，可以提高支架的力学性能和生物学性能。Objective To investigate the effects of arginine-glycine-aspartic acid （RGD）-containing peptides and epoxy ehloropropane （EC） on decellular porcine vavle scaffolds. Methods GRGDSPC peptide was immobilized with EC on the deeelluarized valve scaffolds, and prepared by trypsinase and TritonX-100. Three groups were created ： deeellular scaffold group, EC-treated group, and EC ＋ RGD-treated group. Mechanical properties of the different groups were compared respectively. Then, mesenchymal stem cells （MSCs） from rats were seeded. Results Ultimate tensile strength values for porcine decellualr scaffolds treated with EC （785.8 ± 13.2） g/mm^2 appeared to be relatively greater than group EC （628.4 ± 5.3） g/mm^2, and deeellular scaffold group [ （621.2± 7.9 ） g/mm^2, P 〈 0.05 ]. After MSCs seeding, MTF test showed that more cells attached on the valve scaffolds treated with RGD ＋ EC group （0.72 ± 0.16） then EC-treated group （0.41 ± 0. 10）, and decellular seaffold group （0.47 ± 0. 14, P 〈 0.05 ）. Histological investigation showed that cells of decelluarized valve scaffolds in RGD ＋ EC-treated group were richer. Conclusion It is an effective method for decellularized valve scaffolds modification with RGD peptides and EC to improve mechanical properties,cell adhesion and proliferation.

关 键 词：精氨酸 甘氨酸 天冬氨酸 细胞黏附 组织工程 心脏瓣膜 

分 类 号：R318.08[医药卫生—生物医学工程]