湖北麦冬花药愈伤组织诱导及再生植株的获得  

Callus induction and plant regeneration of Liriope spicata var. prolifera anther

作　　者：周群[1] 周健丘[1] 王小刚[1] 王菁菁[1] 李敬文[1] 陈家春[1] 

机构地区：[1]华中科技大学同济药学院天然药物化学与资源评价湖北省重点实验室,湖北武汉430030

出　　处：《中国中药杂志》2009年第17期2160-2162,共3页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2006BAI06A15-4);高等学校博士学科点专项科研基金项目(20060487071)

摘　　要：目的：探讨药用植物湖北麦冬花药愈伤组织诱导和植株再生条件。方法：以湖北麦冬花药为外植体，采用MS培养基，附加不同的植物激素进行实验。常规压片法结合显微镜进行再生植株染色体的计数分析。结果：MS＋2，4-D 1.0 mg·L^-1＋KT 2.0 mg·L^-1诱导愈伤组织效果最好，愈伤组织诱导率可达41.07%。MS＋6-BA 1.5～2.0 mg·L^-1＋NAA 0.1～0.3 mg·L^-1适于不定芽的诱导，不定芽转入附加NAA 0.1～0.3 mg·L^-1的1/2 MS的生根培养基上，生根后获得完整的再生植株，再生植株为体细胞起源。同时，讨论了4 ℃低温预处理对愈伤组织诱导的影响。结论：建立了湖北麦冬花药体细胞组织培养体系和快速繁殖途径。Objective： To study the technique of the callus induction from anther and plant regeneration of medicinal plants Liriope spicata var. prolifera. Method： Callus was induced from anther of L. spicata var. prolifera on a MS medium supplemented with different hormones.The squash methods combined with a microscope were used to analyze chromosomes of regenerated plantlets. Result： MS＋2,4-D 1.0 mg·L^-1＋KT 2.0 mg·L^-1 gave the highest induction ratio which was 41.07%.MS＋6-BA 1.5-2.0 mg·L^-1＋NAA 0.1-0.3 mg·L^-1 was suitable for the induction and proliferation of indefinite buds.The buds were transferred to 1/2 MS medium supplemented with NAA 0.1-0.3 mg·L^-1 for rooting.The shoots produced roots of culture and formed complete plantlets.The regenerated plantlets originated from somatic cells. At the same time,the effects of pretreatment of low temperature at 4 ℃ on the callus induction were studied and discussed.Conclusion： This paper sets up the method of tissue culture of anther somatic-cells and intermediate propagation of L. spicata var. prolifera.

关 键 词：湖北麦冬 花药培养 愈伤组织 植株再生 

分 类 号：S567.232[农业科学—中草药栽培]