银杏内酯B柔性纳米脂质体的制备及体外透皮研究  

Preparation and transdermal diffusion of flexible nanoliposomes of ginkgolid B

作　　者：刘婷[1] 甄小龙[1] 程昊[1] 李伟泽[2] 郝保华[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]中国药科大学药学院,江苏南京210009

出　　处：《中国中药杂志》2009年第17期2181-2184,共4页China Journal of Chinese Materia Medica

摘　　要：目的:制备银杏内酯B(GB)柔性纳米脂质体,并对其体外透皮规律进行研究。方法:采用薄膜分散法制备银杏内酯B柔性纳米脂质体,并对其形态及粒径大小进行分析;采用改良的Franz扩散池进行体外透皮吸收试验,比较银杏内酯B醇溶液、银杏内酯B柔性纳米脂质体及普通银杏内酯B纳米脂质体的经皮累积渗透量及渗透速率。结果:此方法制得的脂质体平均包封率为(89.52±1.76)%,平均粒径为(208.3±25.49)nm,Zata电位为-49.2 mV。柔性纳米脂质体8 h的累积透过量为189.97μg.cm-2,8 h的渗透速率为23.75μg.cm-2.h-1。结论:柔性纳米脂质体包封率较高,稳定性良好,可显著促进银杏内酯B的透皮吸收。Objective： To prepare flexible nanoliposomes of Ginkgolid B （GB） and study the transdermal diffusion law. Method： Flexible nanoliposomes of GB were prepared by film dispersion method, and the shape and particle size of nanoliposomes were analyzed. GB permeation tests in vitro through the skin of rats were performed in modified Franz diffusion cell. The accumulated permeation amounts of GB alcoholic solution, flexible nanoliposomes of GB and ordinary nanoliposomes of GB were compared. Result： The average encapsulation percentage , the particle size, and the Zata potential of the flexible nanoliposomes were （89.52±1.76）%,and was（208.3±25.49）nm,and was -49.2 mV, respectively. The accumulated permeation amount of flexible nanoliposomes in 8 hours was 189.97 μg·cm-2, and its transdermal permeability in 8 hours was 23.75 μg·cm-2·h-1. Conclusion： The encapsulation percentage of the flexible nanoliposomes is good,and the stability of the GB nanoliposomes is fine. Flexible nanoliposomes can significantly enhance the diffusion rate of GB through the skin of rats.

关 键 词：银杏内酯B 柔性纳米脂质体 体外透皮 

分 类 号：TQ461[化学工程—制药化工]