三七对大鼠肺组织内CYP和GST基因表达的影响  

Effect of Panax notoginseng on genes expression of CYP and GST in lung tissues of rats

作　　者：杨秀芬[1] 廖梅春[1] 杨子明[1] 郭菁菁[1] 高倩倩[1] 

机构地区：[1]广西中医学院药学院药理学教研室,广西南宁530001

出　　处：《中国中药杂志》2009年第17期2236-2240,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30660228);广西自然科学基金项目(桂科基0731058)

摘　　要：目的:研究三七对大鼠肺组织内细胞色素P450(cytochrome P450,CYP)和谷胱甘肽S-转移酶(glutathione S-trans-ferase,GST)基因表达的影响。方法:雄性SD大鼠,三七2,4 g.kg-1,每日1次连续灌胃14 d,用逆转录-实时定量PCR法检测肺组织内的CYP1A1,CYP1A2,CYP1B1,CYP2B1,CYP2E1,CYP3A1,CYP4A1基因和谷胱甘肽-S转移酶GST-m1,GST-pi基因的表达情况。结果:与空白组相比,三七不影响肺组织内CYP2E1,CYP1A2和GST-pi基因的表达,但2 g.kg-1的三七可诱导CYP3A1基因的表达(4.00倍,P<0.05),2,4 g.kg-1的三七可分别诱导GST-m1基因的表达(分别是1.64倍,P<0.05和1.53倍,P>0.05)和CYP1A1基因的表达(3.44倍,P>0.05和6.05倍,P<0.05),2,4 g.kg-1的三七对CYP1B1基因(0.81倍,P>0.05和0.38倍,P>0.05)有抑制趋势;2,4 g.kg-1的三七明显抑制CYP2B1基因(0.47倍,P<0.05和0.50倍,P<0.05)和CYP4A1基因(0.54倍,P<0.05和0.72倍,P<0.05)的表达。结论:三七对肺组织不同的CYP基因和不同的GST基因的影响有明显的差异,这对临床合理使用三七和研究三七的作用机制具有重要意义。Objective： To examine the effects of Panax notoginseng on the expression of cytochrome P450 （CYP） genes and glutathione S-transferase （GST） genes in lung tissues of male SD rats. Method： Rats were administered P. notoginseng 2 or 4 g·kg-1 bw/d by gavage daily for 15 days. The levels of gene expression of CYP1A1, CYP1A2, CYP1B1, CYP2B1, CYP2E1, CYP3A1, CYP4A1 and glutathione S-transferase m1 （GST-m1） and glutathione S-transferase pi （GST-pi） were examined by quantitative real-time reverse-transcription polymerase chain reaction （quantitative real time-RT-PCR） assays. Result： The expression of CYP2E1, CYP1A2 and GST-pi genes was not changed by P. notoginseng treatment, however, 2 g·kg-1 dose of P. notoginseng gave a 4.00-fold （P〈0.05） induction of CYP3A1 mRNA. P. notoginseng significantly increased mRNA expressions of GSTm1 （1.64-fold, P〈0.05 and 1.53-fold, P〉0.05） and CYP1A1 （3.44-fold, P〉0.05 and 6.05-fold, P〈0.05） in the 2 g·kg-1 and 4 g·kg-1 bw/d treatment groups, respectively, but P. notoginseng had a inhibitory tendency on mRNA expressions of CYP1B1 （0.81-fold, P〉0.05 and 0.38-fold, P〉0.05） and significantly inhibited the expressions of CYP2B1 （0.47-fold, P〈0.05 and 0.50-fold, P〈0.05） and CYP4A1 （0.54-fold, P〈0.05 and 0.72-fold, P〈0.05） genes in the two groups. Conclusion： A specific effect of P. notoginseng on the expression of different cytochrome P-450 genes or glutathione S-transferase genes in the lung tissues of rats was observed in this investigation. These findings would be very important and helpful for studying the mechanism of action of P. notoginseng and its reasonable use in clinic.

关 键 词：三七 细胞色素P450 谷胱甘肽S-转移酶 基因表达 

分 类 号：R285.5[医药卫生—中药学]