血管紧张素-(1-7)对大鼠肾系膜细胞TGF-β_1/Smad/CTGF通路的影响  

Effect of angiotensin-(1-7) on the TGF-β1/Smad /CTGF pathway of cultured glomerular mesangial cell

作　　者：贺红焰[1] 何进[2] 李隽[2] 王明勇[3] 刘建[2] 

机构地区：[1]泸州医学院病理教研室,四川泸州646000 [2]泸州医学院肾病内科,四川泸州646000 [3]泸州医学院分子与免疫实验室,四川泸州646000

出　　处：《中国现代医学杂志》2009年第16期2405-2408,共4页China Journal of Modern Medicine

基　　金：四川省科技厅应用基础研究资助项目(No:05JY029-103-2)

摘　　要：目的探讨血管紧张素-(1-7)[Ang-(1-7)]对大鼠肾系膜细胞TGF-β1/Smad/CTGF通路的影响。方法采用WST法检测培养系膜细胞(GMC)增殖;RT-PCR法检测细胞内信号传递分子Smad3/7及结缔组织生长因子(CTGF)基因mRNA的表达。结果与对照组比较,Ang-(1-7)、明显抑制TGF-β1诱导的GMC增殖(P<0.05),同时,Ang-(1-7)对TGF-β1诱导的系膜细胞内Smad3、Smad7及CTGF基因mR-NA表达有明显的抑制作用(P<0.05)。结论Ang-(1-7)对TGF-β1诱导的系膜细胞增殖有抑制作用,其机制可能与抑制TGF-β1/Smad/CTGF通路有关。[Objective] To investigate the effect of Angiotensin-（1-7） [Ang-（1-7）] on the TGF-β1/Smad/CTGF pathway of Cultured Glomerular Mesangial Cell. [Methods] Cell proliferation were evaluated by WST assay, and Smad3/7, CTGF mRNA expression in GMC were analyzed by RT-PCR. [ Results ] Compared with control groups, Ang-（1-7） obviously inhibit cultured GMC proliferation induced by TGF-β1 （P 〈0.05）;and also inhibit the mRNA expression of Smad3/7and CTGF induced by TGF-β1 in cultured GMC （P 〈0.05）. [Conclusion] Ang-（1-7） can inhibit the proliferation of GMC induced by TGF-β1 and the mechanisms may be associated with effects of suppressing TGF-β1/Smad/CTGF pathway.

关 键 词：血管紧张素一(1-7) 转化生长因子一β1 细胞增殖 Smad3/7 结缔组织生长因子 

分 类 号：R692[医药卫生—泌尿科学] R-332[医药卫生—外科学]