不同玉米品种对氯嘧磺隆的耐药性差异及其机制  

Drug resistance and mechanism between different maize varieties to chlorimuron-ethyl

作　　者：郭玉莲[1,2] 陶波[2] 翟喜海[1] 潘亚清[1] 宋伟丰[1] 李明[1] 

机构地区：[1]黑龙江省农业科学院农药应用研究中心,哈尔滨150086 [2]东北农业大学农学院,哈尔滨150036

出　　处：《植物保护学报》2009年第4期366-370,共5页Journal of Plant Protection

基　　金：国家科技支撑计划(2007BAD87B03)

摘　　要：采用玉米沙培法测定9种玉米品种对氯嘧磺隆的敏感性。结果显示,屯玉88和丰合10对氯嘧磺隆的耐药性较强,IC50值分别为23.98μg/kg和17.83μg/kg;东甜3号的IC50值为0.48μg/kg,对氯嘧磺隆最敏感。氯嘧磺隆对活体ALS的抑制作用远低于离体试验结果,离体条件下不同来源的ALS对氯嘧磺隆的敏感性差异不明显;活体条件下耐药性品种屯玉88和中等耐药品种本育9的ALS活力分别是离体条件下的2.50和2.19倍,而敏感品种东甜3号、东农248和东农888的ALS活力分别是离体条件下的1.02～1.45倍。不同玉米品种ALS和GSTs活性存在差异,耐药性品种ALS和GSTs的活性大于敏感品种。此外,氯嘧磺隆可以诱导GSTs活性增加。This paper determined the drug resistance of 9 maize varieties to chlorimuron-ethyl with sand culture experiment. The results showed that IC50 value for Tunyu 88 and Fenghe 10 was 23.98 μg/kg and 17.83 μg/kg respectively, the tolerance was strong. Dongtian 3 was sensitive to chlorimuron-ethyl. The IC50 was 0.48 μg/kg. The inhibition of chlorimuron-ethyl to ALS in vivo was much lower than in vitro. In vitro, there was no significant difference in the drug resistance of different sources ALS to chlorimuronethyl. In vivo, tolerance varieties ALS activity Tunyu 88 and Benyu 9 respectively was 2.5 and 2. 19 times than those in vitro, but sensitive varieties Dongtian 3 and Dongnong 248 and Dongnong 888 was 1.02 -1.45 times than those in vitro, there was significant difference in maize varieties ALS and GSTs activities, the activities of tolerant varieties AI.S and GSTs higher than sensitive varieties. The chlorimu- ron-ethyl induced GSTs activities increased.

关 键 词：玉米 氯嘧磺隆 敏感性 谷胱甘肽S-转移酶 耐药性 

分 类 号：S451.2[农业科学—植物保护]