蒙古绵羊输卵管上皮细胞培养体系的建立  

Establishment of culture system for growing fallopian tube epithelial cells in Mongolian sheep

作　　者：唐博[1,2] 白萨日娜[2,3] 李淑凤[2] 曹贵方[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [3]内蒙古医学院基础医学院,内蒙古呼和浩特010018

出　　处：《中国兽医杂志》2009年第8期3-6,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(30460093)

摘　　要：为了建立蒙古绵羊输卵管上皮细胞培养体系作为良好的体外研究模型,本试验取蒙古绵羊输卵管,运用0.25%胰蛋白酶消化的方法分离上皮细胞进行体外培养。对培养细胞进行形态学、免疫组织化学方法鉴定。原代培养细胞用胰蛋白酶/EDTA方法成功传代,传三代后检测其染色体核型。结果显示,成功获得了原代和传代培养细胞,经鉴定为上皮细胞,传三代后经染色体核型分析显示细胞核型正常,培养的细胞健康状况良好,可以用做体外研究模型。In order to establish the culture system for growing fallopian tube epithelial cells in Mongolian sheep as an in vitro culture model, we used 0.25 % trypsin/EDTA digestion method to obtain the pri mary and passage epithelial cells of the fallopian tubes of Mongolian sheep. The cells after three passages were identified as epithelial cells by morphological techniques and immunohistochemical methods. The status of passage epithelial cells was identified by Karyotype examination. The results showed that the primary and passage epithelial cells of the fallopian tubes of Mongolian sheep can be obtained, and can be used in vitro culture.

关 键 词：上皮细胞培养 绵羊 免疫组化 核型分析 

分 类 号：S851.3[农业科学—预防兽医学]