化浊轻身颗粒的质量标准研究  

Study on Qualitative and Quantitative Methods for Huazhuqingshen Granules

作　　者：吴炜[1] 

机构地区：[1]安徽省亳州市人民医院,安徽亳州236803

出　　处：《中国实验方剂学杂志》2009年第8期24-26,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：研究化浊轻身颗粒中主要药物的鉴别和含量测定方法。方法：采用薄层层析鉴别主要药物,用高效液相色谱法测定制剂中四羟基二苯乙烯苷的含量。结果：建立了制剂中黄芪、大黄、何首乌的鉴别方法,四羟基二苯乙烯苷的线性范围为0.164 8-3.296 0μg（r=0.9996）;加样回收率为99.0%,（RSD为3.0%,n=6）。结论：该实验建立的鉴别和含量测定方法专属性强、重复性好,可用于该制剂的质量控制。Objective：To study the gualitative and quantitative methods of identification and assay Huazhuqingsheng Granules.Methods： The major compounds in this preparation were identified by the thin layer chromatographic methods and the content of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside was determined by high performance liquid chromatography.Results： Rhubarb、Fleecetlower root and milkvetch root were identified.The linear range of 2,3,5,4′-tetrahydroxystilbene-2-Ο-β-D-glucoside was within（0.164 8）～（3.2960 μg）（r=（0.999 6）.）The average recovey（n=6） was 99.0%（RSD=3.0%）.Conclusion： The identification and determination methods established were specific,and repeatable.It could be used to control the quality of this preparation.

关 键 词：化浊轻身颗粒 薄层鉴别 四羟基二苯乙烯苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]