油井采出液中微生物群落结构的T-RFLP分析  

MICROBIAL COMMUNITY STRUCTURE IN PRODUCTION WATER FROM A DAQING PETROLEUM RESERVOIR

作　　者：吴亚嫚[1] 谷峻[1] 谭燕[1] 池昌桥[2] 汤岳琴[2] 吴晓磊[1,2] 

机构地区：[1]清华大学环境科学与工程系,环境模拟与污染控制国家重点实验室,北京100084 [2]北京大学能源与资源系,北京100871

出　　处：《土壤学报》2009年第5期878-886,共9页Acta Pedologica Sinica

基　　金：国家重点基础研究发展规划(973)项目(2005CB221308);国家自然科学基金项目(30570033)资助

摘　　要：应用末端标记限制性片段长度多态性(Terminal Restriction Fragment Length Polymorphism,T-RFLP)和克隆文库分析,以微生物群落16SrRNA基因(16S rDNA)为目标,对大庆油田过渡带油井采出液(于2005年7月和10月取样)中的微生物群落结构进行了解析和比较。T-RFLP分析表明,2005年7月和10月油井采出液中古菌群落结构较为单一,随时间变化不大;而细菌群落结构较为复杂,不同时间群落中的优势菌有明显的差别。古菌和细菌16S rDNA片段测序和系统发育分析表明,大庆油田过渡带油井采出液古菌群落中的优势菌均为产甲烷菌;细菌群落中的优势菌则与β、γ、δ、ε变形杆菌(Proteobacteria)、拟杆菌(Bacte-roidetes)和脱铁杆菌(Deferribacteres)有较高的相似性,细菌群落中检出了大量的未培养微生物(Deep-branching lineages)。Structure of a production water microbial community in a Daqing petroleum reservoir was investigated by T-RFLP （Terminal Restriction Fragment Length Polymorphism） and clone libraries approaches. Small subunit rRNA gene （ 16S rDNA） was retrieved from the samples sampled in July and October 2005. T-RFLP analyses were conducted with the results that the arehaeal community was less diverse than the bacterial community. Time change resulted in the shift of microbial community structure; however, the structure of the archaeal Community was not remarkably different between July and October 2005. In contrast, the patterns of bacterial community showed significant difference in these two months. The arehaeal 16S rDNA partial sequences were mainly affiliated with Methanosarcinales and Methanomicrobiales. The bacterial phylotypes were very closely related to the ＂Betaproteobacteria Gammaproteobacteria Deltaproteobacteria Epsilonproteobacteria＂, Bacteroidetes, and Deferribacteres as well as a deep-branching microbial cluster which was predominant microorganisms in the production water from October 2005.

关 键 词：T—RFLP 油井采出液 16S RDNA 微生物群落 产甲烷菌 

分 类 号：TE357[石油与天然气工程—油气田开发工程] Q93[生物学—微生物学]