二氧化硫吸入对小鼠肺和心组织DNA-蛋白质交联作用的影响  

Study on DNA-protein Crosslink in Lung and Heart of Mice Induced by SO_2 Inhalation

作　　者：解静芳[1,2] 刘丽娜[2] 金国文[2] 毕明丽[1] 周晶[1] 孟紫强[2] 

机构地区：[1]山西大学环境与资源学院,山西太原030006 [2]山西大学环境医学与毒理学研究所,山西太原030006

出　　处：《环境与职业医学》2009年第4期362-365,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助项目(编号:20677035;30740037)

摘　　要：[目的]研究二氧化硫(SO2)吸入致小鼠肺、心组织蛋白质氧化损伤和对DNA-蛋白质交联(DPC)率的影响。[方法]分别用浓度为14、28、56mg/m3的SO2气体对小鼠动态染毒7d(每天6h),对照组小鼠在同样饲养条件下吸入新鲜空气。用2,4-二硝基苯肼(DNPH)比色法测定蛋白质羰基(PCO)含量,用KCl-SDS法测定DPC率。[结果]SO2各染毒浓度组与对照组相比,均可致小鼠肺、心PCO含量和DPC率升高。雌性小鼠SO2染毒浓度与肺、心PCO含量的回归方程分别为:y=0.1744x+4.192(R2=0.9998),y=0.0420x+3.896(R2=0.9763);雄性小鼠分别为:y=0.0441x+4.204(R2=0.9945),y=0.1553x+4.488(R2=0.9883),均呈现明显的剂量-效应关系。雌性小鼠SO2染毒浓度与肺、心DPC率回归方程分别为:y=0.0395x+1.404(R2=0.9913),y=0.0121x+1.614(R2=0.9902);雄性小鼠分别为:y=0.0132x+1.616(R2=0.9821),y=0.0329x+1.45(R2=0.9922),同样呈现明显的剂量-效应关系。[结论]SO2吸入可致小鼠肺、心PCO含量和DPC率呈剂量依赖性升高。随着SO2浓度的增加,对肺、心蛋白质的氧化损伤加剧,DPC率升高。吸入同浓度SO2小鼠的肺、心PCO含量和DPC率结果表明,对肺的损伤远大于心。[ Objective ] To study the effects of inhaling SO2 on protein oxidative damage and protein-DNA crosslink reaction mechanism in lung and heart of mice. [ Methods ] The mice were treated with SO2 of different concentrations （ 14, 28, 56 mg/m^3 ）for 6 h/d and the control group was treated with fresh air under the same conditions. The protein carbonyl（ PCO ）contents were measured by spectrophotometrie DNPH assay, and the DNA-protein crosslink （ DPC ） coefficients were measured by KCI-SDS assay. [ Results ] The results shewed that PCO and DPC contents in lung and heart of female and male mice inereased in the group of 14-56 mg/m^3 in a dose（ SO2 ）-dependent manner. The PCO dose-effect eurves could be fitted well with the equations y=0.1744x＋4.192（R^2=0.9998）, y=0.1553x＋4.488（R^2=0.9883） and y=0.0441x＋4.204（R^2=0.9945）, y=0.0420x＋3.896（R^2=0.9763）for female and male mice, respectively. The DPC dose-effect curves could be fitted well with the equations y=0.0395x＋1.404（R^2=0.9913）,y=0.0121x＋1.614（R^2=0.9902） and y=0.0132x＋1.616（R^2=0.9821）,y=0.0329x＋1.45（R^2=0.9922） for female and male mice, respectively. These results indicated that the protein oxidative damage and the formation of DPC induced by SO2 depended on the concentration of SO2. [ Conclusion ] As the concentration of See rises, the protein oxidative damage becomes more serious, and the DPC levels also rise. In comparison with heart of mice, the damage of SO2 to lung is much more serious.

关 键 词：SO2 肺和心组织 蛋白质羰基 DNA-蛋白质交联 

分 类 号：R114[医药卫生—卫生毒理学]