锰对大鼠纹状体谷氨酸代谢的影响  

Effect of Manganese on the Glutamate Metabolism in Rat's Striatum

作　　者：徐斌[1] 徐兆发[1] 邓宇[1] 高健[1] 邓小强[1] 辛辛[1] 

机构地区：[1]中国医科大学公共卫生学院环境卫生学教研室,辽宁沈阳110001

出　　处：《环境与职业医学》2009年第4期378-380,共3页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金(编号:30771834)

摘　　要：[目的]观察锰对大鼠脑谷氨酸代谢的影响。[方法]Wistar大鼠24只,按体重随机分成4组,每组6只。第1组为对照组,皮下注射0.9%氯化钠,第2～4组为锰染毒组,分别皮下注射8、40、200μmol/kg的氯化锰溶液,注射容量均为5mL/kg,染毒4周,每周5次。测定脑纹状体谷氨酰胺酶(PAG)、谷氨酰胺合成酶(GS)活性和谷氨酸(Glu)、谷氨酰胺(Gln)含量以及细胞凋亡率。[结果]染锰4周后,随着染锰剂量的增加,PAG活性逐渐升高,而GS活性逐渐下降;同时Glu含量也逐渐升高,Gln含量逐渐下降;脑纹状体细胞凋亡率明显升高(P<0.01)。[结论]锰可以通过增强PAG活性和抑制GS活性,使Glu-Gln循环代谢紊乱,产生神经毒性。[ Objective ] To observe the effect of manganese on the glutamate metabolism in rats striatum. [ Methods ] A total of 24 Wistar rats were divided into four groups by weight at random. The first group was the control group in which 0.9% NaCl was injected subcutaneously. The second to fourth groups were test groups in which 8, 40,200 μmol/kg MnCl2 respectively were injected subcutaneously with the capacity of each injection 5 mL/kg of body weight. 4 weeks after administration, the rats were sacrificed to get the tissue samples of their striatum. The activities of phosphate-activated glutaminase（ PAG ）, glutamine synthetase （ GS ）, the levels of glutamatic acid（ Glu ）, glutamine（ Gln ）and the ratio of apoptosis in rats striatum were determined. [ Results ] Compared with the control group, the PAG activity was increased and GS activity was decreased in the groups administered with Mn （ P 〈 0.01 ）. Moreover, Glu level was increased and Gln level was decreased in the group administered with Mn（ P 〈 0.01 ）. As the Mn dose injected increased, the ratio of apoptosis in rats striatum was also increased（ P 〈 0.01 ）. [ Conclusion ] Exposure to Mn in rats would influence the glutamate metabolism and induced apoptosis in rats striatum by activating PAG and restraining GS.

关 键 词：锰 谷氨酸 神经毒性 

分 类 号：R994.6[医药卫生—毒理学]