大孔吸附树脂分离纯化番石榴叶总皂苷的工艺研究  

Separation and Purification of Total Saponins from Guava Leaf with Macroporous Absorptive Resin

作　　者：马连荣[1] 黄丽秀[1] 欧丽兰[1] 田芳年[1] 莫肖云[1] 黄锁义[2] 

机构地区：[1]右江民族医学院 [2]右江民族医学院药学系,广西百色53300

出　　处：《化工技术与开发》2009年第8期4-6,共3页Technology & Development of Chemical Industry

基　　金：右江民族医学院2007-2009年度普高学生科研项目立项资助课题(右医科字[2008]4号)

摘　　要：研究大孔吸附树脂分离纯化番石榴叶总皂苷的工艺条件。番石榴叶用70%乙醇回流提取后,上D101型大孔树脂,水洗后分别用30%,50%,70%乙醇洗脱,以番石榴叶总皂苷的洗脱率为指标,考察大孔树脂分离纯化番石榴叶总皂苷的吸附性能和洗脱参数。番石榴叶总皂苷主要富集于30%、50%乙醇洗脱液部分,大孔吸附树脂的吸附容量为17.53 mg.g-1,洗脱率达70.42%,而50%乙醇洗脱时总皂苷纯度可达55.68%,优选洗脱条件为用水洗去水溶性杂质,50%乙醇洗脱总皂苷。A method for the separation and purification of total saponins from guava leaf by column chromato- graphy of macroporous absorptive resin was established. Radix clematidis was extracted with 70% ethanol and then the extraction was purified with I^01 maeroporous absorptive resin. After eluting with distilled water and 30% ,50% and 70% ethanol respectively, the absorption capability and elution parameters of macroporous ab- sorptive resin were measured by the indexes of elution rate and refinement rate of total saponins. Total saponins of guava leaf were enriched in the 30 % , 50 % ethanol eluted solution fraction. The elution rate of maeroporous absorptive resin was 70.42%, the absorption capability was 17.53 mg/g,and the purity of total saponins of gua- va leaf was up to 55.68 %. The optimum elution condition was： removing water-soluble impurity by water, elut- ing total saponins with 50 % ethanol.

关 键 词：大孔吸附树脂 番石榴叶 总皂苷 提取工艺 

分 类 号：R284[医药卫生—中药学]