TAK1siRNA对C2C12细胞内TAK1基因表达的影响  

Effect of TAK1 siRNA on TAK1 Gene Expression in C2C12 Cells

作　　者：黄志清[1] 陈小玲[1] 陈代文[1] 

机构地区：[1]四川农业大学动物营养研究所,四川雅安625014

出　　处：《生物技术》2009年第4期9-12,共4页Biotechnology

基　　金：国家重点基础研究发展计划"973"项目(No.2004CB117506)资助

摘　　要：目的:高效下调TAK1基因表达的小干扰RNA(siRNA)分子的获得。方法:采用脂质体转染方法,将3对(siRNA ID#94455s、iRNA ID#94549s、iRNA ID#189006)人工合成的TAK1基因特异的小干扰RNA(siRNA)分子分别导入小鼠成肌细胞C2C12中,采用实时荧光定量PCR方法分析细胞内TAK1基因的相对表达。结果:和对照组相比,siRNA ID#94455s、iRNA ID#94549和siRNA ID#189006分别下调了细胞内TAK1基因的mRNA表达水平33.34%、46.73%和79.97%。结论:实验获得了能够高效下调TAK1基因表达的siRNA。Objective： To obtain the highly effective TAK1 siRNA. Method： Mouse myoblast C2C12 cells were transfected with the synthesized TAK1 - specific small interfering RNA （siRNA） duplexes, siRNA ID# 94455, siRNA ID# 94549, and siRNA ID# 189006, respectively, using Lipofectamine 2000. The TAK1 mRNA abundance was determined using real- time quantitative PCR.Result： The expression levels of TAKI mRNA in the cells transfected with siRNA ID# 94455, ID# 94549, and ID# 189006 were decreased 33.34%, 46.73%, and 79.97%, respectively, compared to the negative control siRNA. Conclusion： The siRNA which can effectively down- regulate TAK1 gene expression was obtained in this study.

关 键 词：TAK1 SIRNA C2C12 实际荧光定量PCR 

分 类 号：Q786[生物学—分子生物学]