猪胸膜肺炎放线杆菌flic蛋白二级结构与B细胞表位预测  

Prediction of Secondary Structure and B Cell Epitope for flic of Actionobacillus pleuropneumoniae

作　　者：李任峰[1] 田香勤[2] 何启盖[3] 赵坤[1] 姜金庆[1] 胡建和[1] 王三虎[1] 

机构地区：[1]河南科技学院动物科学学院,河南新乡453003 [2]新乡医学院医学研究中心,河南新乡453003 [3]华中农业大学动物医学院农业微生物学国家重点实验室动物病原分室,湖北武汉430070

出　　处：《生物技术》2009年第4期13-15,共3页Biotechnology

基　　金：河南省基础与前沿研究项目(082300430020);河南科技学院青年创新基金项目(20065053)资助

摘　　要：目的:预测和分析猪胸膜肺炎放线杆菌(Actionobacillus pleuropneumoniae,APP)鞭毛蛋白(flic)的二级结构和B细胞表位。方法:利用生物信息学方法对flic基因推导的氨基酸序列进行结构特征和B细胞表位预测分析。结果:flic蛋白为非稳定型脂蛋白,含有2个酪蛋白激酶Ⅱ磷酸化位点(39-42 SirD,164-167 SvkD)和3个蛋白质激酶C磷酸化位点(39-41 SiR,161-163 TsR、164-166 SvK)。该蛋白无信号肽,在21Ala^38Ala处存在跨膜区的可能性最大。flic蛋白的二级结构主要由无规卷曲区域、α-螺旋和β-折叠组成,而形成较少的转角结构。该蛋白的B细胞表位可能位于55~61和152~158区段内或其附近区域。结论:预测结果将有助于确定flic蛋白的B细胞表位,为进一步研究flic基因功能及研制APP基因工程疫苗提供参考。Objective：To predict the secondary structure and B cell antigenic epitopes of App flie. Method： Based on APP flie amino acid sequence, the secondary structure was predicted by GOR4,PHD and SOPMA; β- turn, accessibility, flexibility, anfigenicity and hydrophilicity index were predicted by Chou- Fasman, Emini, Kolaskar and Parker respectively. Result： The results showed that flic was of a non- stable lipoptptein, two casein kinase Ⅱ phosphorylation site and three protein kinase C phosphorylation site were located in No.39 -42 SirD, 164- 167 SvkD and 39 - 41SIR, 161 - 163TsR, 164 - 166SvK respectively, the most possible B cell epitopes of file were located in or adjacent to amino acid 55 - 61 and 152 - 158. All the predicted epitopes were located in random coil frame of the protein for the secondary structure. Conclusion： These results provided reference and theory basis for research on function of file and developing genetieally engineering vaccine for APP.

关 键 词：猪胸膜肺炎放线杆菌 二级结构 B细胞抗原表位 

分 类 号：Q518.1[生物学—生物化学]