代谢工程改造运动发酵单胞菌用于提高乙醇产量  

Metabolic Engineering of Zymomonas mobilis to Improve It's Fermentation of Ethanol

作　　者：王茜[1] 李海燕[1] 苏志国[2] 熊平[1] 汪浩勇[1,2] 

机构地区：[1]湖北工业大学湖北省工业微生物重点实验室,湖北武汉430068 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190

出　　处：《生物技术》2009年第4期30-34,共5页Biotechnology

基　　金：湖北省优秀中青年团队计划项目("构建乙醇高温发酵基因工程菌";T200705)资助

摘　　要：目的:采用可以在运动发酵单胞菌中表达的操纵子构建重组运动发酵单胞菌,用于提高该细菌对高温高糖的耐受性和提高乙醇产量。方法:用外来的YfdZ、MetB和Hsp构建的多顺反子质粒,转化运动发酵单胞菌而使其获得新的代谢途径。在玉米水解液中,验证了该多顺反子质粒对运动发酵单胞菌产生乙醇的影响。结果:与对照菌相比,在37℃和糖浓度为28%的培养条件下,该基因工程菌的乙醇产量提高到183.2%。在37℃,糖浓度为28%并添加氮源的条件下,该基因工程菌的乙醇产量提高到148.0%。结论:YfdZ、MetB及Hsp三种基因的共同作用能显著提高运动发酵单胞菌的乙醇产量和发酵温度。Objective： Y fdZ gene, MetB gene and Hsp gene from other source were introdue, ed into Zymomonas mobilis to improve its ethanol produetion at high sugar eoneentration. Method： Zymomonas mobilis was transformed by YfdZ- MetB - Hsp multi - eistron. Ethanol production by the reeombinant strain was analyzed in eorn hydrolyzate media. Result： The produetion of ethanol of the metabolie engineered Zymomonas mobilis was 1.83 times more than it＇s wild type. counterpart in eorn hydi＇olyzate with a sugar coneentration as 28% at 37℃. And the production of ethanol of the metabolic engineered Zymomonas mobilis was 1.48 times more than it＇s wild type eoanterpart in eoro hydrolyzate with a sugar concentration as 28% and a nitrogen concentration as 0.1% at 37℃. Conclusion： The combination of YfdZ, MetB and Hsp can significantly improve the fermentation temperature and ethanol production of Zymomonas mobilis.

关 键 词：运动发酵单胞菌 YfdZ MetB HSP 乙醇产量 高糖 

分 类 号：Q785[生物学—分子生物学]